Effect of Si Junziwan on Expression of Lon Protein in Hippocampal CA3 Region of SAMP8 Mice
10.13422/j.cnki.syfjx.20221836
- VernacularTitle:四君子丸对SAMP8小鼠海马CA3区神经元Lon蛋白表达的影响
- Author:
Xudong LIU
1
;
Lu REN
1
;
Dan MA
1
;
Songnan WANG
1
;
Huaxin YU
1
;
Lingzhi WANG
1
;
Huihui LIU
1
;
Danyu ZHAO
1
;
Dehong SHAN
1
Author Information
1. Liaoning University of Traditional Chinese Medicine， Shenyang 110847， China
- Publication Type:Journal Article
- Keywords:
Si Junziwan;
Alzheimer's disease;
Lon protein;
hippocampus;
mitochondrion
- From:
Chinese Journal of Experimental Traditional Medical Formulae
2022;28(24):35-41
- CountryChina
- Language:Chinese
-
Abstract:
ObjectiveTo study the expression changes of Lon protein and mitochondrial dynamics-related protein in the hippocampus of SAMP8 mice and provide a theoretical basis for the treatment of Alzheimer's disease by invigorating the spleen and supplementing Qi. MethodEight 3-month-old SAMR1 mice were used as the normal group， and 32 3-month-old SAPM8 mice were divided into model group， western medicine group （0.013 g·kg-1）， low-dose Si Junziwan group （3.24 g·kg-1）， and high-dose Si Junziwan group （12.56 g·kg-1）， with 8 mice in each group. The western medicine group was gavaged with donepezil， and the Si Junziwan low- and high-dose groups were gavaged with Si Junziwan for 30 days. The positioning navigation experiment of the water maze was started on the 25th day， and the space exploration experiment of the water maze was started on the 30th day. On the 30th day， the protein expression of mitofusin 2 （MFN2） was detected by immunohistochemistry， the expression of adenosine 5'-monophosphate （AMP）-activated protein kinase （AMPK） was detected by enzyme-linked immunosorbent assay （ELISA）， the content of ATP was detected by colorimetry， the microstructure of neuron mitochondria was detected by electron microscope， and the expressions of Aβ protein， Lon protein， dynamin-related protein 1 （DRP1） protein， and MFN1 protein were detected by Western blot. ResultAs compared with the normal group， the latency escape period increased， the number of crossings decreased， the expression of AMPK increased， and the content of ATP decreased in the model group. The expressions of Aβ protein and DRP1 protein increased （P<0.01）， whereas the expressions of Lon protein， MFN1 protein decreased in the model group （P<0.05，P<0.01）， and MFN2 protein decreased. The vacuolation of mitochondria increased and the cristae broke in the model group. As compared with model group， the time of the latent escape period decreased （P<0.01）， and the number of crossings increased in the low-dose and high-dose Si Junziwan groups （P<0.05）. The expression of AMPK （P<0.01） decreased， the content of ATP increased （P<0.01）， the expression of Aβ and DRP1 protein decreased （P<0.05， P<0.01）， and the expression of MFN1 protein was up-regulated （P<0.05） in high-dose Si Junziwan groups. The vacuolation was more obvious in the low-dose Si Junziwan group， whereas the vacuolation was restored and the ridge was clear in the high-dose Si Junziwan group. ConclusionSi Junziwan treats Alzheimer's disease by up-regulating the protein expression of Lon， correcting the disorder of mitochondrial division and fusion protein， and changing the memory function of SAMP8 mice.
