Mechanisms of Danggui Shaoyaosan in Improving Cognitive Ability in SAMP8 Mice by Regulating Gut Microbiota via 16S rDNA Sequencing
10.13422/j.cnki.syfjx.20221909
- VernacularTitle:基于16S rDNA测序研究当归芍药散调控肠道菌群改善SAMP8小鼠认知能力的作用机制
- Author:
Xiaoping TIAN
1
;
Jun XIA
1
;
Jingwen WEI
2
;
Wei PENG
1
;
Wenying HUAI
1
;
Yu YOU
1
;
Tiane ZHANG
1
;
Jiayuan ZHANG
1
;
Wei HUANG
1
;
Yunhui CHEN
1
Author Information
1. Chengdu University of Traditional Chinese Medicine,Chengdu 611137,China
2. Sichuan University,Chengdu 610041,China
- Publication Type:Journal Article
- Keywords:
Alzheimer's disease;
Danggui Shaoyaosan;
SAMP8;
gut microbiota
- From:
Chinese Journal of Experimental Traditional Medical Formulae
2022;28(24):26-34
- CountryChina
- Language:Chinese
-
Abstract:
ObjectiveTo analyze the effects of Danggui Shaoyaosan (DSS) on the gut microbiota of the Alzheimer's disease (AD) model in SAMP8 mice based on 16S rDNA sequencing. MethodTwenty-four SAMP8 mice aged seven months were randomly divided into low-, medium-, and high-dose DSS groups (14.4, 28.8, 57.6 g·kg-1·d-1) and a model group according to a random number table, with six rats in each group. Six SAMR1 mice of the same age were assigned to the normal group. After intragastric administration for eight consecutive weeks, 16S rDNA sequencing was performed to detect the gut microbiota of feces in mice. Morris water maze was employed to assess the directional navigation and space exploration ability of mice. Nissl staining was performed to observe the pathological changes of neurons in the hippocampal CA1 area. Enzyme-linked immunosorbent assay (ELISA) was adopted to measure the protein content of hippocampal amyloid β-protein (Aβ) and hyperphosphorylated Tau (p-Tau). ResultCompared with the normal group, the model group presented a declining α diversity (P<0.05), markedly altered β diversity, prolonged escape latency (P<0.05), reduced number of platform crossings and cumulative duration in the targeted quadrant (P<0.05), decreased neurons and Nissl bodies in the CA1 hippocampal area, and up-regulated Aβ and p-Tau expression (P<0.05). However, DSS intervention enhanced the α diversity, and medium- and high-dose DSS, especially the medium-dose DSS, could result in α diversity similar to the control group. Moreover, at the phylum level, the abundance of Firmicutes increased (P<0.05), while the abundance of Bacteroidetes and Proteobacteria decreased (P<0.05). At the genus level, the abundance of Lactobacillus and other genera increased (P<0.05), while the abundance of Bacteroides, Helicobacterium, Rikenella, Parabacteroides, Sutterella, and Mucilaginibacter decreased (P<0.05). The DSS groups also showed shortened escape latency (P<0.05), increased number of platform crossings and cumulative duration in the targeted quadrant (P<0.05), increased Nissl bodies (P<0.05), and reduced Aβ and p-Tau content (P<0.05). Pearson correlation analysis showed that the abundance of Mucilaginibacter, Bacteroides, and Sutterella was negatively correlated with the cognitive ability of SAMP8 mice, while the abundance of Lactobacillus and Butyricimonas was positively correlated with the cognitive ability of SAMP8 mice. ConclusionDSS can improve the cognitive ability of SAMP8 mice, and its mechanism may be related to the regulation of gut microbiota diversity and community composition.