Simultaneous determination of seven components in Qutan Zhike granules by QAMS
10.16438/j.0513-4870.2022-0811
- VernacularTitle:一测多评法同时测定祛痰止咳颗粒中7种成分的含量
- Author:
Jia-yi CHEN
1
;
Jian-ming LI
1
;
Li-hong GU
1
;
Hui-chan HOU
1
;
Jing-you LI
2
Author Information
1. NMPA Key Laboratory for Quality Evaluation of Traditional Chinese Medicine, Guangzhou Institute for Drug Control, Guangzhou 510160, China
2. Guangzhou Huacheng Pharmaceutical Company Limited, Guangzhou 510555, China
- Publication Type:Research Article
- Keywords:
Qutan Zhike granule;
relative correction factor;
quantitative analysis of multi-components with single marker;
external standard method;
two-point correction method
- From:
Acta Pharmaceutica Sinica
2022;57(11):3405-3410
- CountryChina
- Language:Chinese
-
Abstract:
We have established a quantitative analysis of multi-components by single marker method (QAMS) for the simultaneous determination of apigenin-7-glucuronide, quercitrin, yuankanin, luteolin, apigenin, hydroxygenkwanin, and genkwanin in Qutan Zhike Granules. The chromatographic column used was an Agilent EC-C18 (150 mm × 4.6 mm, 4 μm), the mobile phase was methanol-0.15% phosphoric acid solution (gradient elution), and the detection wavelength was 338 nm. Apigenin was chosen as the internal reference standard, the relative correction factors for the six components were determined by multi-point correction method and included apigenin-7-glucuronide, quercitrin, yuankanin, luteolin, hydroxygenkwanin and genkwanin. According to the two-point correction method combined with the relative retention time correction of the components to be tested, the peak location was determined. The contents of these seven compounds in 10 batches of Qutan Zhike Granules samples were determined with relative correction factors, and the relative error (RE) was used to compare the results to that of the external standard using the External Standard Method to verify the accuracy of QAMS. The relative correction factors for apigenin-7-glucuronide, quercitrin, yuankanin, luteolin, hydroxygenkwanin and genkwanin were 1.762 8, 2.310 4, 1.898 4, 1.282 8, 1.191 3 and 1.066 9, respectively. RSDs of the relative correction factors were all lower than 3%. The peaks for each constituent were accurately located by the two-point correction method combined with relative retention time correction, and the predicted retention time was close to the actual retention time. The relative error of the contents by QAMS and determined by ESM in 10 batches of Qutan Zhike Granules were between -5% and 5%. This content determination method can be used for the simultaneous determination of seven components in Qutan Zhike Granules.
