Identification of quality markers associated with the anti-inflammatory effects of Jiangzhenxiang, a folk medicine
10.16438/j.0513-4870.2022-0796
- VernacularTitle:民间药材降真香抗炎质量标志物的研究
- Author:
Miao QIN
1
;
Wan-na XIONG
2
;
Jin-mei HUANG
1
;
Xiao LIU
2
;
Ying ZHAO
2
;
Fang LI
1
,
3
;
Ying-jian LI
4
Author Information
1. College of Pharmacy, Guangxi University of Chinese Medicine, Nanning 530200, China
2. Department of Pharmacy, Guangxi Medical College, Nanning 530023, China
3. Key Laboratory of Common Technology of Traditional Chinese Medicine Preparation, Nanning 530200, China
4. Forestry College of Guangxi University, Nanning 530004, China
- Publication Type:Research Article
- Keywords:
Jiangzhenxiang;
italic>Dalbergia benthamii Prain;
anti-inflammatory;
Q-marker;
isorhamnetin
- From:
Acta Pharmaceutica Sinica
2022;57(11):3396-3404
- CountryChina
- Language:Chinese
-
Abstract:
We have identified anti-inflammatory quality markers (Q-markers) of Jiangzhenxiang. The chemical components of Jiangzhenxiang were identified by mass spectrometry and the substances that contribute to its anti-inflammatory activity, their targets and signaling pathways were analyzed by network pharmacology to identify potential Q-markers of the anti-inflammatory action of Jiangzhenxiang. The potential Q-markers were verified by high performance liquid chromatography, and in vitro experiments verified the anti-inflammatory activity and the target of the potential Q-markers. The experimental scheme was approved the Guangxi University of Chinese Medicine Institutional Animal Ethical and Welfare Committee. The results show that 31 chemical components were identified by mass spectrometry from the Jiangzhenxiang extract. Through network pharmacological screening, 727 component targets, 422 disease targets and 110 targets including prostaglandin G/H synthase 2 (PTGS2) were obtained. These targets were mainly enriched in 498 biological processes including inflammatory response and response to lipopolysaccharide, with 101 pathways that included the TNF signaling pathway, toll-like receptor signaling pathway and others. Isorhamnetin, formononetin, naringenin, glycitein, ursolic acid and oleanolic acid were detected by high performance liquid chromatography. Jiangzhenxiang medicated serum and 6 components thereof could significantly reduce the content of nitric oxide, interleukin-6 (IL-6) and tumor necrosis factor-α in RAW264.7 cells induced by lipopolysaccharide (P < 0.01 or P < 0.05). These six components are regarded as the potential anti-inflammatory Q-markers of Jiangzhenxiang. Isorhamnetin was screened and verified from the 6 potential Q-markers as an inhibitor of PTGS2 by molecular docking and in vitro cyclooxygenase 2 (COX-2) activity assay. The half-inhibitory concentration of isorhamnetin was 9.55 μmol·L-1. In summary, extracts of Jiangzhenxiang showed significant in vitro anti-inflammatory actions. The anti-inflammatory mechanism of Jiangzhenxiang appears to be related to regulation of TNF signaling pathway and Toll-like receptor signaling pathway meditated by IL-6, RAC alpha serine/threonine protein kinase, PTGS2 and other targets. Isorhamnetin, formononetin, naringenin, glycitein, ursolic acid and oleanolic acid could be regarded as the Q-markers of Jiangzhenxiang. Isorhamnetin appears to act as a COX-2 inhibitor.
