Construction of targeted nanoprobe based on hyaluronic acid and its anti-tumor activity in vitro
10.11665/j.issn.1000-5048.20220505
- VernacularTitle:基于透明质酸的靶向纳米探针的构建及体外抗肿瘤活性
- Author:
Xiaoyu QI
1
;
Yufei YE
;
Xuemei CHEN
;
Xiaonan ZHU
;
Yuting ZHANG
;
Jian YIN
;
Xiaoli WANG
;
Nandi ZHOU
Author Information
1. 江南大学生物工程学院糖化学与生物技术教育部重点实验室
- Publication Type:Journal Article
- Keywords:
hyaluronic acid;
doxorubicin;
IR808;
nanoprobe;
breast cancer;
near infrared imaging;
chemotherapy-photodynamic synergistic therapy
- From:
Journal of China Pharmaceutical University
2022;53(5):542-553
- CountryChina
- Language:Chinese
-
Abstract:
Hyaluronic acid (HA) was used as drug delivery carrier, and the doxorubicin (DOX), IR808 and catalase (CAT) were modified on hyaluronic acid (HA) to form the nano-probe CAT@HA-DOX-IR808 NPs with anti-tumor and fluorescence imaging by self-assembly.It was characterized by UV-Vis spectrophotometer, fluorescence spectrophotometer and transmission electron microscope, and its fluorescence imaging and antitumor activity were studied at solution and cell level.The experimental results showed that CAT@HA-DOX-IR808 NPs displayed a uniform near-spherical morphology with a size of 75 nm approximately.Under the condition of pH 5.0 + hyaluronidase (HAase), the release rate of DOX reached more than 80% in the first 10 hours. In the CD44 positive cells, laser confocal imaging results showed that the group of CAT@HA-DOX-IR808 NPs had more significant fluorescence signals than the group of free drugs and negative cell.In the cytotoxicity test, only about 40% of the MDA-MB-231 cells survived at the highest concentration of CAT@HA-DOX-IR808 NPs of the group of CAT@HA-DOX-IR808 NPs + NIR.Therefore, CAT@HA-DOX-IR808 NPs possess significantly enhanced anti-tumor effect with broad application prospect in the imaging and treatment of breast cancer in vitro.
