Optimization of Culture Medium and Fermentation Condition of Pseudomonas ZL8
10.13422/j.cnki.syfjx.20221318
- VernacularTitle:假单胞菌菌株ZL8发酵培养基及发酵条件的优化
- Author:
Min ZHANG
1
;
Yuan ZHENG
1
;
Yuyang ZHAO
1
;
Junhui ZHOU
1
;
Tiegui NAN
1
;
Yuan YUAN
1
Author Information
1. National Resource Center for Chinese Material Media，China Academy of Chinese Medical Sciences，Beijing 100700，China
- Publication Type:Journal Article
- Keywords:
Pseudomonas ZL8;
endophytic fungus of Polyporus umbellatus;
biocontrol bacteria;
optimization of culture medium;
optimization of fermentation conditions
- From:
Chinese Journal of Experimental Traditional Medical Formulae
2022;28(22):174-181
- CountryChina
- Language:Chinese
-
Abstract:
ObjectiveIn the previous stage，our research group isolated Pseudomonas ZL8 from Polyporus umbellatus， which has a broad-spectrum inhibitory effect on plant pathogenic fungi such as Fusarium oxysporum of Salvia miltiorrhiza and promotes plant growth. On this basis， this study optimized the fermentation medium and conditions of Pseudomonas ZL8，to improve the number of bacteria in the fermentation broth per unit volume， and provide a basis for the development and application of this strain. MethodThe growth curve of strain ZL8 in LB culture medium was determined and its growth law in liquid medium was clarified. With the number of live bacteria in the fermentation broth of strain ZL8， the dry weight of the bacteria and the A600 value of the broth as detection indicators， single factor investigation was carried out on different nitrogen sources，carbon sources and inorganic salts to determine the optimal medium components. Additionally， orthogonal test was conducted to optimize the component ratio to screen the optimal fermentation medium. Similarly， single factor investigation and orthogonal test were also carried out in four aspects： inoculation amount，culture temperature，time and rotating speed to obtain the optimal culture conditions. ResultThe optimal medium for liquid fermentation of strain ZL8 was peptone 15 g·L-1， glucose 7.5 g·L-1， and potassium chloride 10 g·L-1. After 48 h of fermentation，the number of viable bacteria in the fermentation liquid could reach 8.93×109 cfu·mL-1，3.93 times that of LB liquid medium （P<0.01）. The optimal fermentation conditions were as follows： inoculation amount 3%， temperature 28 ℃， time 72 h，and rotating speed 220 r·min-1， and the number of viable bacteria in the fermentation broth could reach 6.37×1010 cfu·mL-1. ConclusionCompared with LB culture medium and the initial fermentation conditions，the optimized culture medium and conditions could effectively increase the bacterial volume of strain ZL8 per unit volume and prolong the stable growth of the strain， with low medium cost， which provided a theoretical basis for the efficient fermentation and application of Pseudomonas ZL8.
