Investigation of Metabolic Profile Changes of Fresh Fruit of Schisandrae Chinensis Fructus After Green Removing Based on 1H-NMR Metabonomics
10.13422/j.cnki.syfjx.20220357
- VernacularTitle:基于1H-NMR代谢组学技术考察五味子鲜果采后“杀青”的代谢轮廓变化
- Author:
Lulu NIU
1
;
Wei MA
1
;
Xiuhong WU
1
;
Nannan XING
1
;
Xiaoyan ZHANG
1
;
Shuxiang ZHANG
1
;
Lei LIU
1
Author Information
1. Institute of Traditional Chinese Medicine，School of Pharmacy， Heilongjiang University of Chinese Medicine，Harbin 150040，China
- Publication Type:Journal Article
- Keywords:
Schisandrae Chinensis Fructus;
proton nuclear magnetic resonance （1H-NMR）;
green removing;
metabolomics;
multivariate statistical analysis;
amino acids;
alkaloids
- From:
Chinese Journal of Experimental Traditional Medical Formulae
2022;28(22):167-173
- CountryChina
- Language:Chinese
-
Abstract:
ObjectiveTo find the different metabolites of Schisandrae Chinensis Fructus fresh fruits dried in different ways by proton nuclear magnetic resonance （1H-NMR） metabonomics technology， and to explore the possible effect of green removing on its metabolic profile. MethodFifteen samples of Schisandrae Chinensis Fructus fresh fruits were used， and each 3 samples was divided into one group. The samples were processed by five methods， including drying， drying in the sun， microwave， blanching and steaming. These samples were extracted with 50% methanol and analyzed by 1H-NMR. Combined with multivariate statistical analysis， the differences of the metabolic profile between green removing samples and dried samples， sun-dried samples were compared. ResultA total of 27 chemical components were identified by 1H-NMR and Chenomx database， mainly including amino acids， alkaloids， carbohydrates and organic acids. These metabolites were mainly involved in the energy metabolism of fruit postharvest physiology. The results of multivariate statistical analysis showed that there were significant differences among the 5 groups. The dried and sun-dried samples were used as the controls， 17 differential metabolites （asparagine， citric acid， glucose， sucrose， choline glycerophosphate， trigonelline， alanine， lysine， glycerol， leucine， isoleucine， valine， uridine， threonine， methionine， phenylalanine and fructose） were screened by variable importance in the projection （VIP） value and S-plot. One-way ANOVA was used for comparison between groups， the results showed that compared with dried and sun-dried samples， the contents of sucrose and choline glycerophosphate increased significantly （P<0.05， P<0.01） in samples treated with green removing， but the contents of valine， leucine， isoleucine， threonine， phenylalanine and methionine decreased significantly （P<0.01）， the content of asparagine increased significantly （P<0.05， P<0.01） in samples treated by microwave， while the contents of trigonelline and uridine decreased significantly （P<0.01） in samples treated by blanching. ConclusionThe consumption， decomposition and/or transformation of active ingredients may be inhibited by interfering with its energy metabolism after the green removing of Schisandrae Chinensis Fructus fresh fruits.
