Analysis of Characteristics of Volatile Organic Compounds and Bacterial Communities in Rhizosphere Soil During Growth of Rehmanniae Radix
10.13422/j.cnki.syfjx.20220255
- VernacularTitle:地黄生长过程中根际土壤挥发性有机物与细菌群落特征分析
- Author:
Weifeng LI
1
;
Xiaotong GENG
2
;
Haiyan GONG
1
;
Fengqing WANG
3
;
Jingwei LEI
1
;
Caixia XIE
1
;
Zhongyi ZHANG
4
Author Information
1. Henan Engineering Technology Research Center for Quality Control and Evaluation of Traditional Chinese Medicine，Henan University of Chinese Medicine，Zhengzhou 450046，China
2. Xinyang Agriculture and Forestry University，Xinyang 464000，China
3. Henan Agricultural University，Zhengzhou 450046，China
4. Fujian Agriculture and Forestry University，Fuzhou 350021，China
- Publication Type:Journal Article
- Keywords:
Rehmanniae Radix;
rhizosphere soil;
gas chromatography-mass spectrometry （GC-MS）;
bacterial community characteristics;
volatile organic compounds;
high-throughput sequencing;
continuous cropping obstacle
- From:
Chinese Journal of Experimental Traditional Medical Formulae
2022;28(22):149-158
- CountryChina
- Language:Chinese
-
Abstract:
ObjectiveTo analyze the changes of volatile organic compounds （VOCs） and bacterial community characteristics in rhizosphere soil during the growth of Rehmanniae Radix， as well as the relationship between VOCs and bacterial community structure， so as to lay the foundation for the evaluation of the characteristics of continuous cropping soil and the regulation of continuous cropping soil microorganisms. MethodThe rhizosphere soil during the three main growth periods of Rehmanniae Radix in July， August and October was used as the research object. Gas chromatography-mass spectrometry （GC-MS） was used to determine the relative contents of VOCs in ethyl acetate and dichloromethane parts in rhizosphere soil. The characteristics of rhizosphere bacterial community structure was determined by high-throughput sequencing， SPSS 24， SIMCA 14.1 and other software were used to analyze the differences of VOCs and bacterial community structure in rhizosphere soil of the three periods， and the main VOCs and iconic bacteria that caused the differences were screened， and the correlation between VOCs and bacterial community structure was analyzed. ResultThere were differences in VOCs in different parts of rhizosphere soil during the three growth stages of Rehmanniae Radix. Among them， and the main VOCs that cause differences were dioctyl isophthalate， 2-octyl-1-dodecanol and 2-ethylhexyl p-toluic acid in the ethyl acetate part， and 1，3-benzenedicarboxylic acid， di （2-propylpentyl） phthalate and 2-ethylhexyl p-toluenecarboxylic acid in the dichloromethane part. From the seedling stage to the end of tuber enlargement of Rehmanniae Radix， the relative abundance of dominant bacteria such as Actinobacteria， Firmicutes and Chloroflexi in the rhizosphere soil was gradually decreased， and there were unique bacterial communities in rhizosphere soil of each growth stage. Correlation analysis showed that the VOCs in rhizosphere soil of Rehmanniae Radix had an impact on the rhizosphere bacterial community structure， especially the components of esters and alcohols. ConclusionDuring the growth of Rehmanniae Radix， the characteristics of rhizosphere soil are mainly manifested in the content changes of main VOCs such as esters and alcohols and the gradual decrease of the abundance of the main beneficial bacteria， and the VOCs in rhizosphere soil play a certain role in shaping the structure of bacterial community.
