Pharmacodynamic Material Basis of Hippophae Folium in Treatment of Hyperlipidemia
10.13422/j.cnki.syfjx.20221019
- VernacularTitle:沙棘叶治疗高脂血症的药效物质基础
- Author:
Yu TANG
1
;
Yina ZHANG
1
;
Yuanrong ZOU
1
;
Jiaxin QIAO
1
;
Siyu LIU
1
;
Feng GAO
1
Author Information
1. College of Pharmacy，Shaanxi University of Chinese Medicine， Xianyang 712046， China
- Publication Type:Journal Article
- Keywords:
Hippophae Folium;
hyperlipidemia;
mechanism of action;
molecular docking
- From:
Chinese Journal of Experimental Traditional Medical Formulae
2022;28(22):116-122
- CountryChina
- Language:Chinese
-
Abstract:
ObjectiveTo analyze the pharmacodynamic material basis of Hippophae Folium in the treatment of hyperlipidemia by network pharmacology and experimental verification. MethodThe hyperlipidemic HepG2 cell model induced by oleic and palmitic acid （molar ratio 2∶1） was established. The optimal concentration of Hippophae Folium containing serum was determined by cell counting kit （CCK）-8 method. The cells were intervened by the medicated serum， and oil red O staining was used to determine the success of the model. The contents of total cholesterol （TC） and triglyceride （TG） were determined by enzyme-linked immunosorbent assay （ELISA）. The possible mechanism of action was analyzed by network pharmacology， and molecular docking was performed to detect the binding ability of the potential targets. ResultCCK-8 assay showed that 10% medicated serum was the optimal concentration for cell growth. Oil red O staining proved that the hyperlipidemic cell model induced by oleic and palmitic acid has been built. After treatment with medicated serum， the contents of TG and TC decreased， indicating that Hippophae Folium had a good therapeutic effect on hyperlipidemia. Network pharmacology revealed that the core targets of Hippophae Folium in the treatment of hyperlipidemia were albumin （ALB）， peroxisome proliferative activated receptor γ （PPARγ） and matrix metalloprotein（MMP）-9， involving 755 biological processes， 73 molecular functions and 3 cellular components. By Kyoto Encyclopedia of Genes and Genomes（KEGG） analysis， it was found PPAR， hypoxia inducible factor（HIF）-1， AMP-activated protein kinase（AMPK） and other signal pathways were involved in the treatment of hyperlipidemia by Hippophae Folium. ConclusionHippophae Folium containing serum （10%） could reduce lipid accumulation and intracellular TG and TC levels in hyperlipidemic cell model， and its mechanism of action might be realized by activating PPAR signal pathway.
