Effect of Didang Xianxiong Decoction on Myocardium Ultrastructure and Expression of CTGF and Its Receptor LRP in Diabetic Rats
10.13422/j.cnki.syfjx.20221202
- VernacularTitle:抵当陷胸汤对糖尿病大鼠心肌超微结构和CTGF及其受体LRP蛋白表达的影响
- Author:
Zhengyin CAI
1
;
Jun CHU
1
;
Quangen CHU
1
;
Yun XUAN
1
;
Jie CHENG
1
;
Pan WANG
1
Author Information
1. Anhui University of Chinese Medicine，Hefei 230038，China
- Publication Type:Journal Article
- Keywords:
Didang Xianxiong decoction;
diabetic rats;
cardiomyocytes;
connective tissue growth factor （CTGF）;
low-density lipoprotein receptor-related protein （LRP）
- From:
Chinese Journal of Experimental Traditional Medical Formulae
2022;28(22):100-105
- CountryChina
- Language:Chinese
-
Abstract:
ObjectiveTo observe the effect of Didang Xianxiong decoction on the ultrastructure of myocardium and the expression of connective tissue growth factor （CTGF） and low-density lipoprotein receptor-related protein （LRP） in diabetic rats. MethodThe diabetic rat model was established by the intraperitoneal injection of high-dose streptozotocin （55 mg·kg-1） and the rats were further fed for 3 weeks. Forty model rats were randomly assigned into model group， a Xiao Xianxiongtang （4.05 g·kg-1·d-1） group， Xuefu Zhuyutang （6.30 g·kg-1·d-1） group， Didang Xianxiong decoction （8.10 g·kg-1·d-1） group， and alagebrium chloride （ALT-711， 3 mg·kg-1·d-1） group， with 8 rats in each group. Ten normal healthy rats were randomly selected as the blank group. The corresponding drugs were administrated by gavage， and the blank group and the model group were given distilled water at a dose of 10 mL·kg-1·d-1 for 8 weeks. The myocardial tissue was collected from the rats under anesthesia at the end of the 8th week for pathological examination. The expression of CTGF and its receptor LRP in the myocardial tissue was detected by immunohistochemistry and Western blot. ResultCompared with the blank group， the modeling led to obvious ultrastructural damage of the myocardium， up-regulated the expression of CTGF （P<0.01）， and did not significantly change the expression of LRP. Compared with the model group， the drugs alleviated the damage in myocardium ultrastructure， down-regulated the expression of CTGF （P<0.01）， and did not significantly change the expression of LRP. Moreover， Didang Xianxiong decoction showed better performance than Xiao Xianxiongtang and Xuefu Zhuyutang （P<0.01）. ConclusionDidang Xianxiong decoction may protect myocardial tissue by down-regulating the high expression of CTGF in myocardial tissue of diabetic rats， thereby delaying myocardial fibrosis. The results indicate that the therapy of treating both phlegm and blood stasis has better performance than the simple method of resolving phlegm or stasis.
