Simiaowan Up-regulates Intestinal ABCG2 Expression to Promote Intestinal Uric Acid Excretion in Hyperuricemia Rats
10.13422/j.cnki.syfjx.20221601
- VernacularTitle:四妙丸上调高尿酸血症大鼠小肠ABCG2表达促进肠道尿酸排泄的作用
- Author:
Yongqi ZHANG
1
;
Jiewei CHEN
1
;
Bowen YE
2
;
Zehan HAO
1
;
Hao DAI
2
Author Information
1. Shanghai University of Traditional Chinese Medicine （TCM），Shanghai 201203，China
2. Guanghua Hospital Affiliated to Shanghai University of TCM，Shanghai 200052，China
- Publication Type:Journal Article
- Keywords:
Simiaowan;
hyperuricemia;
intestinal uric acid;
ATP-binding cassette superfamily G （White） member 2 （ABCG2）
- From:
Chinese Journal of Experimental Traditional Medical Formulae
2022;28(22):33-39
- CountryChina
- Language:Chinese
-
Abstract:
ObjectiveTo observe the effect of Simiaowan on the intestinal ATP-binding cassette superfamily G （White） member 2 （ABCG2） expression and the intestinal uric acid excretion in hyperuricemia rats. MethodA total of 48 SD male rats were randomized into the normal， model， benzbromarone （4.7 mg·kg-1）， and high-， medium-， low-dose Simiaowan groups （2 260.6， 1 130.3， 565.2 mg·kg-1， respectively）， with 8 rats in each group. Potassium oxonate and hypoxanthine was employed to induce hyperuricemia in rats （21 days）. On the 8th day， administration began （once a day for 14 days）. Rats were killed on the 21st day， and serum uric acid， serum creatinine， blood urea nitrogen， and intestinal uric acid were detected. The protein expression of ABCG2 in the small intestine was detected by Western blot. The ABCG2 protein expression and localization in intestinal tissues were determined by immunohistochemistry. The ABCG2 mRNA expression in small intestine was measured by quantitative real-time PCR. ResultThe levels of serum uric acid， serum creatinine， and blood urea nitrogen in the model group were higher than those in the normal group （P<0.01）. Low level of serum uric acid in the three Simiaowan groups and benzbromarone group （P<0.01）， high level of intestinal uric acid in medium-dose and low-dose Simiaowan groups （P<0.05， P<0.01）， high level of serum creatinine in benzbromarone group （P<0.01）， and low level of blood urea nitrogen in low-dose Simiaowan group （P<0.05） were observed as compared with those in the model group. Serum uric acid showed insignificant difference between the low-dose Simiaowan group and benzbromarone group. The expression of ABCG2 protein in the model group was lower than that in the normal group （P<0.05）. The expression of ABCG2 protein in the medium-dose and low-dose Simiaowan groups （P<0.05， P<0.01）， the high-dose Simiaowan group， and benzbromarone group increased as compared with that in the model group. ABCG2 mRNA expression was insignificantly different between the model group and the normal group， while the expression in the medium-dose and low-dose Simiaowan groups was higher than that in the model group （P<0.05）. ABCG2 protein was mainly distributed in intestinal villi， and ABCG2 protein expression demonstrated no significant difference between the model group and the normal group. The ABCG2 protein expression in the three Simiaowan groups increased as compared with that in the model group （P<0.05）. ConclusionSimiaowan can significantly reduce the serum uric acid level in hyperuricemia rats. Particularly， the low-dose Simiaowan shows similar efficacy to benzbromarone in lowering uric acid and protects renal function. The mechanism is the likelihood that it up-regulates intestinal ABCG2 expression to promote intestinal excretion of uric acid.
