Shenghuitang Mitigates Cognitive Impairment and Circadian Rhythm Disturbance in Mouse Model of Alzheimer's Disease via Regulating Expression Levels of Neurotransmitters
10.13422/j.cnki.syfjx.20221940
- VernacularTitle:生慧汤通过调节神经递质改善阿尔茨海默病模型小鼠认知损伤和昼夜节律紊乱
- Author:
Qinghua LONG
1
;
Binbin ZHAO
2
;
Li DING
2
;
Ping WANG
2
Author Information
1. Medical School， Hubei Provincial Key Laboratory of Occurrence and Intervention of Rheumatic Diseases， Hubei Minzu University， Enshi 445000， China
2. Institute of Geriatrics， Hubei University of Chinese Medicine， Wuhan 430065， China
- Publication Type:Journal Article
- Keywords:
Alzheimer's disease;
Shenghuitang;
neurotransmitter;
learning and memory;
circadian rhythm
- From:
Chinese Journal of Experimental Traditional Medical Formulae
2022;28(22):16-22
- CountryChina
- Language:Chinese
-
Abstract:
ObjectiveTo observe the effect of Shenghuitang on serum levels of neurotransmitters in the mouse model of Alzheimer's disease （AD） and explore the mechanism of Shenghuitang in mitigating the cognitive impairment and circadian rhythm disturbance of AD. MethodTwenty-seven APP/PS1 dementia mice were randomly assigned into a model group， a donepezil （0.92×10-4 g·kg-1·d-1） group， and a Shenghuitang （13.5 g·kg-1·d-1） group. Another nine wild-type C57BL/6JNju mice was set as the control group. The mice were administrated with corresponding drugs by gavage and the control and model groups were given the same volume of pure water. Every group was continuously treated for 4 weeks. The cognitive function and circadian rhythm of mice were evaluated by Morris water maze test and open field test. The liquid chromatography-tandem mass spectrometry （LC-MS/MS） was employed to determine the expression levels of acetylcholine （ACh）， choline acetyltransferase （ChAT）， norepinephrine （NE）， epinephrine （E）， glutamate （Glu）， 5-hydroxyindoleacetic acid （5-HIAA）， and dopamine （DA） in the serum. ResultCompared with the control group， the modeling increased the escape latency， swimming distance， time of first arrival on the platform， activity time of light environment， activity time of dark environment， and total activity time （P<0.01）， while it decreased the number of crossing the platform and the swimming time in the target quadrant （P<0.01）. Compared with the model group， donepezil and Shenghuitang decreased the escape latency， swimming distance， time of first arrival on the platform， activity time of light environment， activity time of dark environment and total activity time （P<0.05， P<0.01）， while they increased the number of crossing the platform and the swimming time in the target quadrant （P<0.05， P<0.01）. Compared with the control group， the modeling down-regulated the expression levels of ACh and ChAT in the serum （P<0.01）. Compared with the model group， donepezil and Shenghuitang up-regulated the expression levels of ACh and ChAT in the serum （P<0.05， P<0.01）. Compared with the control group， the modeling up-regulated the expression level of Glu in the serum （P<0.01） and down-regulated the expression levels of NE， 5-HIAA， and DA （P<0.01）. Compared with the model group， Shenghuitang down-regulated the expression level of Glu （P<0.05） and up-regulated the expression levels of NE， 5-HIAA， and DA （P<0.05， P<0.01）. The expression levels of NE， Glu， 5-HIAA， and DA in the donepezil group did not change significantly compared with those in the model group. The expression level of E showed no significant difference among different groups. ConclusionShenghuitang may ameliorate the cognitive impairment and circadian rhythm disturbance of AD mice by regulating the levels of neurotransmitters in the serum.
