Effect of Xiaoyaosan on Gastrointestinal Motility in Rats with Liver Depression and Spleen Deficiency Syndrome Based on RhoA/ROCK Signaling Pathway
10.13422/j.cnki.syfjx.20221401
- VernacularTitle:基于RhoA/ROCK信号通路探讨逍遥散对肝郁脾虚证大鼠胃肠动力的影响
- Author:
Ai CHEN
1
;
Yulin ZHENG
1
;
Fang WANG
1
;
Lin GAO
1
;
Xiaohong LI
1
;
Liqiang YANG
1
Author Information
1. Guangxi University of Chinese Medicine，Nanning 530200，China
- Publication Type:Journal Article
- Keywords:
Xiaoyaosan;
liver depression and spleen deficiency syndrome;
stomach;
RAS homologous gene family member A （RhoA）/Rho-related coiled protein kinase （ROCK）
- From:
Chinese Journal of Experimental Traditional Medical Formulae
2022;28(22):1-6
- CountryChina
- Language:Chinese
-
Abstract:
ObjectiveTo observe the effect of Xiaoyaosan on the expression of RAS homologous gene family member A （RhoA） and Rho-related coiled protein kinase 1 and 2 （ROCK1 and ROCK2） in gastric tissues of rats with liver depression and spleen deficiency syndrome and to explore its mechanism of regulating gastrointestinal motility in rats with liver depression and spleen deficiency syndrome. MethodSeventy-two male SD rats were randomly divided into 6 group， namely the normal group， the model group， the fluoxetine group （0.001 8 g·kg-1）， the Xiaoyaosan high （16.7 g·kg-1）， medium （8.35 g·kg-1） and low-dose （4.175 g·kg-1） group， with 12 rats in each group. In addition to the normal group， the rats in other groups were used to establish the model of liver depression and spleen deficiency syndrome by chronic restraint stress. At the same time， the rats in the normal group and the model group were given normal saline （10 mL·kg-1）， and the rats in each drug group were given corresponding doses of drugs once a day for 21 d. After modeling， the gastric emptying rate and intestinal propulsion rate of rats in each group were measured. The mRNA and protein expressions of RhoA， ROCK1， and ROCK2 in gastric tissues were detected by Real-time quantitative polymerase chain reaction （Real-time PCR） and Western blot. ResultAs compared with the normal group， the gastric emptying rate， intestinal propulsion rate， and the mRNA and protein expressions of RhoA， ROCK1， and ROCK2 in gastric tissues in the model group were lower （P<0.05， P<0.01）. The abnormal changes in the above indexes in the Xiaoyaosan high， medium， and low-dose groups were all improved in varying degrees as compared with the model group （P<0.05， P<0.01）. ConclusionXiaoyaosan improves gastrointestinal motility in rats with liver depression and spleen deficiency syndrome， which may be related to the up-regulation of the RhoA/ROCK signal pathway in gastric tissues.
