Examination of Quality Characteristics of Rehmannia glutinosa Leaves by Different Fixation Methods Based on Fingerprint and Antioxidant Activity
10.13422/j.cnki.syfjx.20220446
- VernacularTitle:基于指纹图谱及抗氧化活性考察不同杀青方法地黄叶的质量特征
- Author:
Jinpeng CUI
1
;
Xiaotong GENG
2
;
Mengjuan CHENG
1
;
Weifeng LI
1
;
Juan ZHANG
1
;
Qingpu LIU
1
;
Jingwei LEI
1
;
Caixia XIE
1
Author Information
1. Henan Provincial Engineering Technology Research Center for Quality Control and Evaluation of Traditional Chinese Medicine，Henan University of Chinese Medicine，Zhengzhou 450046，China
2. Xinyang Agriculture and Forestry University，Xinyang 464000，China
- Publication Type:Journal Article
- Keywords:
Rehmannia glutinosa leaves;
fixation method;
fingerprint;
antioxidant activity;
quality evaluation;
high performance liquid chromatography （HPLC）;
near infrared spectroscopy （NIRS）
- From:
Chinese Journal of Experimental Traditional Medical Formulae
2022;28(23):197-204
- CountryChina
- Language:Chinese
-
Abstract:
ObjectiveTo analyze the effect of different fixation methods on the chemical quality characteristics and antioxidant activity of Rehmannia glutinosa leaves， so as to lay a foundation for the selection of processing technology and quality evaluation of this medicinal materials. MethodR. glutinosa leaves was dried at 55 ℃ after treating by three fixation methods （55 ℃， boiling water， 105 ℃）， and then the fingerprints of R. glutinosa leaves were collected by high performance liquid chromatography （HPLC） and near infrared spectroscopy （NIRS）， and their antioxidant activities were analyzed by the 2，2-biphenyl-1-picrylhydrazyl （DPPH） method. Finally， similarity analysis， principal component analysis （PCA）， orthogonal partial least squares-discriminant analysis （OPLS-DA） and Pearson correlation analysis were used to comprehensively evaluate the quality of R. glutinosa leaves with different fixation methods. ResultThe results of HPLC and NIRS fingerprint analysis indicated that there were differences in the quality characteristics of R. glutinosa leaves by different fixation treatments. The comprehensive score of chemical quality of R. glutinosa leaves by fixation at 55 ℃ was the highest， its average comprehensive score was 2.096， followed by fixation at 105 ℃， and the lowest was fixation with boiling water. The antioxidant activity of sample with fixation at 55 ℃ was the highest， followed by fixation with boiling water. The results of OPLS-DA showed that verbascoside， isoacteoside and catalpol were the main components causing the difference in chemical quality of the leaves from the three treatments， and the three components were positively correlated with the antioxidant activity of R. glutinosa leaves. Among them， the correlation between verbascoside and antioxidant activity was extremely significant， and the isoacteoside was significant. ConclusionThe chemical quality and antioxidant activity of R. glutinosa leaves are affected by the method of fixation， and the fixation at low temperature is the best primary processing method of R. glutinosa leaves.
