Comparison Between Integrated and Traditional Decoction Pieces of Pinelliae Rhizoma Praeparatum cum Zingibere et Alumine Based on Quality and Pharmacological Effect
10.13422/j.cnki.syfjx.20220354
- VernacularTitle:基于质量和药理效应比较姜半夏一体化饮片与传统饮片
- Author:
Caixia TAO
1
;
Yukun ZHENG
1
;
Qipan JIAN
1
;
Yuhang SHU
1
;
Jiyuan TU
1
;
Yanju LIU
1
;
Guangzhong WANG
1
Author Information
1. Pharmacy Faculty，Hubei Engineering Technology Research Center of Chinese Medicine Processing，Hubei University of Chinese Medicine，Wuhan 430065，China
- Publication Type:Journal Article
- Keywords:
Pinelliae Rhizoma Praeparatum cum Zingibere et Alumine;
integrated processing;
index components;
high performance liquid chromatography （HPLC）;
anti-inflammatory;
irritation;
traditional Chinese medicine decoction pieces
- From:
Chinese Journal of Experimental Traditional Medical Formulae
2022;28(23):174-181
- CountryChina
- Language:Chinese
-
Abstract:
ObjectiveTo compare the consistency and difference between the integrated decoction pieces （IDP） and traditional decoction pieces （TDP） of Pinelliae Rhizoma Praeparatum cum Zingibere et Alumine （PRPZA） in the component content and pharmacological effect， so as to explain the rationality of the integrated production of PRPZA. MethodThin layer chromatography （TLC）， extract determination， ultraviolet spectrophotometry and high performance liquid chromatography （HPLC） were used to analyze and determine the TLC identification， the contents of water-soluble extract， alum residue， needle-like calcium oxalate crystal， protein， total alkaloids， polysaccharides and three nucleosides （inosine， guanosine and adenosine） of IDP and TDP of PRPZA， and the statistical comparison was made. The anti-inflammatory effect and irritation of IDP and TDP of PRPZA were compared by xylene-induced mouse auricle swelling test and rabbit conjunctival irritation test. ResultCompared with the TDP of PRPZA， the contents of alum residue， needle-like calcium oxalate crystal， protein， polysaccharides， inosine， guanosine and the total amount of three nucleosides in the IDP of PRPZA decreased by 16.95%， 21.27%， 23.78%， 4.74%， 52.12%， 0.24% and 26.04%， the contents of water-soluble extract， total alkaloids and adenosine increased by 7.62%， 114.83% and 125.42%， respectively. IDP and TDP of PRPZA had obvious inhibitory effects on ear edema in mice， but there was no significant difference between them， indicating that the anti-inflammatory effect was consistent. The two decoction pieces of PRPZA had no irritation to the conjunctiva of rabbits， and the difference was not statistically significant between them， suggesting that the safety was similar. ConclusionThere is a certain difference in the component content between the IDP and the TDP of PRPZA， but their anti-inflammatory and irritant effects are similar， and the quality of IDP is slightly better than TDP， which provides a reference for the industrial production and clinical application of the IDP of PRPZA.
