Effect of Yinxing Mihuan Oral Solution on Neovascularization and Vascular Remodeling in Chemical Photothrombosis-induced Focal Cerebral Ischemia Mouse Model
10.13422/j.cnki.syfjx.20222237
- VernacularTitle:银杏蜜环口服溶液对化学光栓塞型局灶性脑缺血小鼠模型微血管生成与重构的影响
- Author:
Mingjiang YAO
1
;
Wei ZHANG
1
;
Bin YANG
1
;
Wenting SONG
1
;
Guangrui WANG
1
;
Yimin WANG
2
;
Yong WANG
2
;
Shaoqing HE
2
;
Jianxun LIU
1
Author Information
1. Beijing Key Laboratory of Pharmacology of Chinese Materia Medica， Institute of Basic Medical Sciences of Xiyuan Hospital， China Academy of Chinese Medical Sciences， Beijing 100091， China
2. Xi'an Buchang Traditional Chinese Cardio-Cerebrovascular Disease Hospital， Xi'an 710082， China
- Publication Type:Journal Article
- Keywords:
Yinxing Mihuan oral solution;
chemical photothrombosis;
focal cerebral ischemia;
neovascularization and vascular remodeling
- From:
Chinese Journal of Experimental Traditional Medical Formulae
2022;28(23):38-43
- CountryChina
- Language:Chinese
-
Abstract:
ObjectiveTo investigate the effects and underlying mechanism of Yinxing Mihuan oral solution （YM） on neovascularization and vascular remodeling in chemical photothrombosis-induced focal cerebral ischemia model in mice. MethodFifty SPF C57BL/6J mice were randomly divided into sham group， model group， ginaton group （12.5 mg·kg-1）， and low- （YM-L， 412 mg·kg-1） and high-dose （YM-H， 824 mg·kg-1） YM groups， with 10 mice in each group. The focal cerebral ischemia model was established by chemical photothrombosis method. Drugs in each group were administered by gavage for 14 consecutive days after operation. The modified neurological severity score （mNSS） and measurement of forelimb grasping were used to evaluate the neurologic impairment of mice. The vascular density of infarct border-zone （IBZ） was measured by fluorescein labelled dextran （FITC-dextran） method. The morphology of IBZ was evaluated and observed by hematoxylin-eosin （HE） staining. The expression of proteins related to neovascularization and vascular remodeling in brain tissues， including vascular endothelial growth factor （VEGF）， platelet and endothelial cell adhesion molecule 1 （CD31）， hypoxia-inducible factor-1α （HIF-1α）， von Willebrand factor （vWF）， and angiogenin （ANG）， was detected by Western blot. ResultCompared with the sham group， the model group showed manifest neurological deficits （P<0.01）， weakened forelimb grasping （P<0.01）， increased vascular density of IBZ （P<0.01）， and obvious pathological changes， such as neuronal necrosis and gliocyte proliferation. After treatment for 14 days， compared with the model group， the YM-H group showed improved neurological deficits （P<0.01）， and the YM-L group and the YM-H group showed strengthened forelimb grasping （P<0.01）. Moreover， the YM-L group displayed increased vascular density of IBZ （P<0.05）， reduced pathological damage， and up-regulated protein expression of CD31， ANG， HIF-1α， and vWF （P<0.05， P<0.01）. ConclusionYM could improve motor function and pathological morphological impairment in chemical photothrombosis-induced focal cerebral ischemia mouse model， and the underlying mechanism might be related to the promotion of neovascularization and vascular remodeling in IBZ.
