Inhibitory Effect of Gancao Fuzitang on Bone Destruction in Collagen-induced Arthritis Mice by Regulating NF-κB Signaling Pathway
10.13422/j.cnki.syfjx.20221605
- VernacularTitle:甘草附子汤调控NF-κB信号通路抑制胶原诱导型关节炎小鼠骨质破坏的作用
- Author:
Kai QIAN
1
;
Xuexia ZHENG
1
;
Haihong LI
2
;
Chen CHEN
2
;
Xinfeng SHEN
2
;
Zhiyi LIAO
2
;
Yiping ZHU
2
;
Chuanming XU
2
;
Dongmei PAN
2
Author Information
1. Guangzhou University of Chinese Medicine，Guangzhou 510405，China
2. Southern Medical University，Guangzhou 510405，China
- Publication Type:Journal Article
- Keywords:
Gancao Fuzitang;
collagen-induced arthritis;
rheumatoid arthritis;
bone destruction;
nuclear factor-κB （NF-κB） signaling pathway
- From:
Chinese Journal of Experimental Traditional Medical Formulae
2022;28(23):1-9
- CountryChina
- Language:Chinese
-
Abstract:
ObjectiveTo explore the mechanism of Gancao Fuzitang （GCFZ）in inhibiting the bone destruction of collagen-induced arthritis （CIA） model in mice. MethodThirty male DBa/1J mice were randomly divided into normal group， CIA group， low-dose GCFZ group （GCFZ-L， 2.4 g·kg-1）， high-dose GCFZ group （GCFZ-H， 4.8 g·kg-1）， and methotrexate group （MTX， 1 mg·kg-1）， with six mice in each group. The CIA model was induced by secondary immunization method. The arthritis index of mice in each group was observed and recorded， and the histopathological changes in ankle joint were observed by hematoxylin-eosin （HE） staining. The damage to ankle cartilage was detected by safranin O-fast green staining. Micro-CT scanning was used to detect the bone destruction of ankle joint， and the expression of nuclear factor-κB p65 （NF-κB p65）， p-NF-κB p65， inhibitory-κB kinase α/β （IKKα/β）， and p-IKKα/β was observed by immunohistochemical staining. ResultCompared with the normal group， the CIA group showed manifest joint swelling and increased arthritis index score （P<0.01）. Compared with the CIA group， the groups with drug intervention could inhibit joint swelling and reduce arthritis index score （P<0.05， P<0.01）. As revealed by HE staining and safranine O-green staining， compared with the CIA group， the groups with drug intervention could inhibit synovial invasion and reduce the destruction of articular cartilage. Micro-CT scanning analysis showed that compared with the CIA group， the GCFZ-H group and the MTX group showed reduced bone destruction scores （P<0.01）. The immunohistochemical results showed that compared with the normal group， the CIA group showed increased optical density values of NF-κB p65， p-NF-κB p65， IKKα/β， and p-IKKα/β（P<0.01）. Compared with the CIA group， the GCFZ-H group and the MTX group showed reduced optical density values of NF-κB p65， p-NF-κB p65， IKKα/β， and p-IKKα/β（P<0.05，P<0.01）. In the GCFZ-L group， only the NF-κB p65 optical density value decreased（P<0.01）. ConclusionGCFZ may inhibit bone destruction in CIA mice by regulating the NF-κB signaling pathway.
