Associations between NLRP3 Levels and Coronary Artery Disease Risk： Mendelian Randomized Study
10.13471/j.cnki.j.sun.yat-sen.univ（med.sci）.2022.0116
- VernacularTitle:NLRP3水平与冠心病风险关联的孟德尔随机化研究
- Author:
Jun-yue YANG
1
;
Si-yu FAN
1
;
Ying-chao TAN
1
;
Hong ZHI
2
;
Li-na WANG
1
Author Information
1. School of Public Health， Southeast University， Nanjing 210009， China
2. ZhongDa Hospital， Southeast University， Nanjing 210009， China
- Publication Type:Journal Article
- Keywords:
NLRP3;
coronary artery disease;
mendelian randomization;
single nucleotide polymorphism
- From:
Journal of Sun Yat-sen University(Medical Sciences)
2022;43(1):133-139
- CountryChina
- Language:Chinese
-
Abstract:
ObjectiveUsing the Mendelian randomization （MR） study design to infer the causal relationship between NLRP3 levels and the risk of CAD. MethodsTotally 321 CAD cases confirmed by coronary angiography（CAG） and 293 normal controls were included in this MR study. Serum NLRP3 levels of all the subjects were determined by ELISA. The polymorphism of NLRP3 gene rs10754558 （C/G） was detected by RFLP-PCR and it was considered as the instrumental variable （Ⅳ） to evaluate the causal relationship between NLRP3 levels and CAD risks. Logistic regression analysis and Linear regression analysis were used to test the association between genotype-phenotype， genotype-disease outcome， and then MR method was used to infer the causal relationship between NLRP3 and CAD risks. ResultsThe association between the NLRP3 gene rs10754558（C/G） variant and the risk of CAD and the level of NLRP3 were statistically significant. The regression coefficients βZY = 0.45 and βZX = 2.34 respectively. The regression coefficient βXY = βZY/βZX = 0.45/2.34 = 0.19， and then it was transformed into OR value （OR=e0.19=1.209）， which meant subjects with the 1 ng/mL increased of NLRP3 level had the 20.9% increased risk of CAD. And also， the traditional case-control study of the NLRP3 levels and CAD risks showed that the subjects with the 1 ng/mL increased of NLRP3 levels were associated with 3.1% increased CAD risk （βXY=0.03， OR=e0.03=1.031， 95%CI=1.003-1.058）， which was a little bit lower than that of MR result. ConclusionsThe inflammasome NLRP3 levels were associated with the increased risk of CAD in a mendelian randomization study and it might be a robust evidence than that of the traditional association study.
