Characterization of Microbiota in Tumor Tissues of Esophageal Squamous Cell Carcinoma
10.13471/j.cnki.j.sun.yat-sen.univ（med.sci）.2022.0316
- VernacularTitle:食管鳞状细胞癌肿瘤组织内菌群检测及特征分析
- Author:
Bo-hong XIAN
1
;
Jun-min WEI
1
;
Gui-bin QIAO
2
;
Xiao-hui CHEN
1
;
Fang-ping XU
3
;
Xin-lan LUO
3
;
Yi-xiang CHEN
1
;
Zi-jun LI
4
Author Information
1. The Second School of Clinical Medicine， Southern Medical University， Guangzhou 510515， China
2. Department of Thoracic Surgery， Guangdong Provincial People′s Hospital， Guangzhou 510080， China
3. Department of Pathology， Guangdong Provincial People′s Hospital， Guangzhou 510080， China
4. Department of Gastroenterology， Guangdong Provincial People′s Hospital， Guangzhou 510080， China
- Publication Type:Journal Article
- Keywords:
esophageal squamous cell carcinoma;
bacteria;
Porphyromonas;
Fusobacterium
- From:
Journal of Sun Yat-sen University(Medical Sciences)
2022;43(3):480-487
- CountryChina
- Language:Chinese
-
Abstract:
ObjectiveTo analyze the difference in esophageal microbiome between the patients with esophageal squamous cell carcinoma （ESCC group） and the healthy controls （HC group）， and to screen out the characteristic bacteria in tumor tissues of patients with esophageal squamous cell carcinoma. MethodsEsophageal tissues were collected from 105 patients with esophageal squamous cell carcinoma and 54 healthy controls. The total DNA of the samples was extracted， and the 16S rDNA V4 region was amplified by PCR， followed by high-throughput sequencing. The data were subjected to OTU clustering and diversity analysis to screen the characteristic bacteria in the ESCC group. Finally， the screening results were to be verified by Q-PCR amplification. ResultsDiversity analysis showed that there were differences in diversity and composition between the ESCC group and the HC group. Species analysis showed that the main components of microbiome in the ESCC group were Fusobacterium， Gemella， Neisseria， Porphyromonas and Streptococcus. While in the HC group the main components of microbiome were Fusobacterium， Actinobacillus， Streptococcus and Prevotella. The LEfSe analysis showed that the expression of porphyrin and Fusobacteria in the ESCC group increased and the difference is statistically significant （P<0.05）. The results of Q-PCR showed that the presence of Porphyromonas gingivalis and Fusobacterium nucleatum in the ESCC group significantly increased. ConclusionsEsophageal cancer has its characteristic flora composition. Porphyromonas gingivalis and Fusobacterium nucleatum in esophageal squamous cell carcinoma tumor tissue are the characteristic bacteria.
