Diagnostic Value of Peripheral Blood Circulating Tumor Cells and Three Tumor Markers in Stage I Endometrial Carcinoma
10.13471/j.cnki.j.sun.yat-sen.univ（med.sci）.2022.0315
- VernacularTitle:外周血循环细胞和三种肿瘤标志物检测对Ⅰ期子宫内膜癌的诊断价值
- Author:
Chun-fang CAI
1
;
Jia-mei GU
2
;
Yan-fei GUAN
3
Author Information
1. Department of Obstetrics and Gynecology, Guangzhou Women and Children's Medical Center, Guangzhou 510120, China
2. Department of Molecular Diagnosis, Sun Yat-sen University Cancer Center, Guangzhou 510060, China
3. Department of Clinical Laboratory, Zhongshan BOAI Hospital Affiliated to Southern Medical University, Zhongshan 528400, China
- Publication Type:Journal Article
- Keywords:
circulating tumor cell;
serum amyloid A;
human epididymal protein 4;
carbohydrate antigen 125;
endometrial carcinoma
- From:
Journal of Sun Yat-sen University(Medical Sciences)
2022;43(3):471-479
- CountryChina
- Language:Chinese
-
Abstract:
ObjectiveTo investigate the diagnostic value of peripheral blood circulating tumor cells （CTC）and three tumor markers in stage I endometrial carcinoma （EC）， so as to provide a scientific basis for finding a noninvasive early diagnosis method of EC. MethodsFrom May 2016 to November 2021， 335 healthy female physical examinees （control group）， 291 patients with benign endometrial lesions （benign lesion group） and 268 patients with stage I EC （EC group） were selected as the research subjects. Samples of 3.2 mL cephalic vein blood were taken from the three groups of subjects composed of the physical examinees and patients before operation. The CTC level was measured by immunofluorescence hybridization and staining. The levels of SAA were measured by latex enhanced immunoturbidimetry， and HE4 and CA125 were measured by electrochemiluminescence. The positive rates and level differences of CTC， SAA， HE4 and CA125 in the three groups were observed. The sensitivity， specificity， coincidence rate， positive predictive value， negative predictive value of CTC， SAA， HE4， CA125， （SAA+HE4+CA125）， （CTC+SAA+HE4+CA125） in the diagnosis of stage I EC were compared Difference between yoden index and receiver operating characteristic （ROC） curve area （AUC）. Results①The positive rates of CTC， SAA， HE4， CA125， （SAA+HE4+CA125）， （CTC+SAA+HE4+CA125） in EC group were higher than those in control group and benign lesion group （P<0.01）. In the benign lesion group， the positive rate of （CTC+HE4+SAA+CA125） was higher than that of other detection indexes. Compared with HE4 and CTC， the difference was statistically significant（χ2=8.58，P=0.003；χ2=33.26， P<0.001）. In EC group， the positive rate of （CTC+HE4+SAA+CA125） was higher than that of other detection indexes. Compared with CTC， SAA， HE4， CA125， （SAA+HE4+CA125）， the difference was statistically significant （P<0.001）. ②The expression levels of CTC， SAA， HE4 and CA125 in EC group were the highest， which was statistically significant compared with the control group and benign lesion group （P<0.001）. ③（CTC+SAA+HE4+CA125） had the highest sensitivity and coincidence rate， negative predictive value and Jordan index. ④The AUC detected by CTC， SAA， HE4， CA125， （SAA+HE4+CA125）， （CTC+SAA+HE4+CA125） were 0.86， 0.81， 0.75， 0.78， 0.82 and 0.95， respectively. Conclusions（CTC+SAA+HE4+CA125） detection has high sensitivity， coincidence rate， negative predictive value， Jordan index and large AUC for the diagnosis of stage I EC. It is a better combination model for the diagnosis of early EC.
