Improvement of the Mouse Model of Chronic Obsessive-compulsive Disorder Induced by Quinpirole
10.13471/j.cnki.j.sun.yat-sen.univ（med.sci）.2022.0309
- VernacularTitle:喹吡罗诱导的慢性强迫症小鼠模型的改进
- Author:
Yu-chong LUO
1
;
Chun-ren WEI
1
;
Xiao CHEN
1
;
Mo-yan LI
1
;
Sheng-lin WEN
1
Author Information
1. Department of Psychiatry， The Fifth Affiliated Hospital of Sun Yat-sen University， Zhuhai 519000， China
- Publication Type:Journal Article
- Keywords:
Quinpirole;
obsessive-compulsive disorder;
obsessive-compulsive model;
mouse
- From:
Journal of Sun Yat-sen University(Medical Sciences)
2022;43(3):412-421
- CountryChina
- Language:Chinese
-
Abstract:
ObjectiveTo obtain a mouse model of chronic obsessive-compulsive disorder with a shorter modeling time and longer symptom duration， we improved an existing method to obtain a simple and stable mouse model of chronic obsessive-compulsive disorder. Methods22 mice were randomly divided into experimental group （n=11） and control group （n=11） . The mice were injected subcutaneously with 0.75 mg/kg quinpirole solution or the same amount of saline in the neck for 19 consecutive days and were put into the compulsive behavior training device. The drinking behavior and drinking volume of the two groups were recorded during training， and the effects of quinpirole on the behavior of mice were evaluated. After training， behavioral studies such as open field test， elevated plus maze test and new object recognition test were conducted to evaluate the anxiety state and cognitive ability of the mice after modeling. In addition， immunofluorescence experiments were performed to explore the neural mechanism of behavioral changes in mice. ResultsQuinpirole injection combined with behavioral training for 19 days could effectively induce compulsive checking behavior without anxiety or cognitive decline in mice （P<0.001）， and this behavior lasted more than 28 days after training. In this model， several brain regions on the cortical-striatum-thalamic-cortical circuit were activated （P<0.000 1）. ConclusionChronic quinpirole administration combined with behavior training can effectively construct a mouse model of OCD with an obvious and stable phenotype， and effectively shorten the time required for modeling.
