Effects of Transcription Factor E2F2 on Cell Adhesion in Multiple Myeloma
10.13471/j.cnki.j.sun.yat-sen.univ（med.sci）.2022.0304
- VernacularTitle:E2F转录因子2对多发性骨髓瘤细胞粘附的影响
- Author:
Zhi-ying MAI
1
;
Shu-na CHEN
1
;
Xing-ding ZHANG
1
;
Wen-jing ZHAO
1
;
Yong-jiang ZHENG
2
Author Information
1. School of Medicine， Sun Yat-sen University， Shenzhen 518107， China
2. Department of Hematology， The Third Affiliated Hospital of Sun Yat-sen University， Guangzhou 510630， China
- Publication Type:Journal Article
- Keywords:
multiple myeloma;
E2F transcription factor 2;
cell adhesion
- From:
Journal of Sun Yat-sen University(Medical Sciences)
2022;43(3):361-372
- CountryChina
- Language:Chinese
-
Abstract:
ObjectiveTo investigate the influence of E2F transcription factor 2 （E2F2） on the cell adhesion of multiple myeloma cells by combining bioinformatics and cellular and molecular experiments. MethodsFirstly， clinical samples and GEO database were used to analyze the relationship between the E2F2 expression and the prognosis of myeloma patients. Then， differential genes were screened from the RNA-seq data of E2F2 knockdown myeloma cell line MM.1S. The GO biological function enrichment and KEGG signal pathway analysis were performed on these differential genes. Meanwhile， the protein interaction network was analyzed by using the string website. The E2F2 stable knockdown MM.1S cell line were constructed， and the expression level of E2F2 was measured by Western Blotting and qRT-PCR. The effect of stable knockdown E2F2 on the adhesion of myeloma cells was detected by cell adhesion experiment， and the expression of cell adhesion related genes were detected by qRT-PCR. The effect of stable E2F2 knockdown on myeloma cell migration was detected by cell migration assay. ResultsThe expression of E2F2 was significantly increased in clinical samples of myeloma patients， and the high expression of E2F2 is correlated with poor prognosis of myeloma patients in the GEO database. The analysis of RNA-seq data revealed 815 differentially expressed genes， of which 508 genes were up-regulated and 307 genes were down-regulated. These genes were closely related to the cell adhesion and angiogenesis. The construction of E2F2 knockdown MM.1S cell lines were verified by Western Blotting and qRT-PCR. Knockdown of E2F2 significantly increased the adhesion level of MM.1S cells and elevated the expression of FN1， PECAM1， ICOSLG and other cell adhesion related genes， while weakening the invasion ability of MM.1S cells， P < 0.05. ConclusionsThrough bioinformatics analysis of the RNA-seq data， we found that the transcription factor E2F2 is closely related to the cell adhesion in myeloma. Knockdown of E2F2 in the MM.1S cell line promotes the expression of cell adhesion related genes， increases the cell adhesion level， and inhibites cell migration ability.
