The Development and Function of Leptomeningeal Lymphatic Endothelial Cells in Mouse
10.13471/j.cnki.j.sun.yat-sen.univ（med.sci）.2022.0301
- VernacularTitle:小鼠软脑膜淋巴管内皮细胞的发育特点及其对行为功能的影响
- Author:
Li-ren TAN
1
;
Song-hua XIAO
1
;
Ming LEI
1
Author Information
1. Department of Neurology， Sun Yat-sen Memorial Hospital， Sun Yat-sen University， Guangzhou， 510120， China
- Publication Type:Journal Article
- Keywords:
leptomeningeal;
lymphatic endothelial cells;
development;
phagocytose;
APP/PS1 mouse;
behavior
- From:
Journal of Sun Yat-sen University(Medical Sciences)
2022;43(3):331-343
- CountryChina
- Language:Chinese
-
Abstract:
ObjectiveTo observe the spatial-temporal distribution of mouse leptomeningeal lymphatic endothelial cells and their effects on behavior. MethodsImmunofluorescence was used to detect the number of Lyve-1+and CD68+ cells in the dorsal， temporal， ventral， and lateral paraventricular leptomeningeal of 1-week， 2-week， 4-week， 10-week， 15-week， and 15-month-old wild-type C57BL mice and 15-week-old APP/PS1 transgenic mice. Two-week-old C57BL mice were randomly grouped as follows： PBS-injected group， anti-Lyve-1-injected group and SAR131675-injected group， which were injected with corresponding reagents into lateral ventricle. Two weeks after injection （i.e.， 4 weeks old）， the three groups of mice were subjected to the open field experiment， the three-chamber social interaction experiment， and the novel object recognition experiment. Then their ratio of leptomeningeal lymphatic cells were detected by immunofluorescence. ResultsThere was no statistical difference in the distribution of leptomeningeal lymphatic endothelial cells in different regions （F=0.8700， P=0. 4668， df=3）. The percentage of Lyve-1+ cells in the leptomeninges of mice decreased with ages （F=17.30， P<0.0001， df=5）. There was no statistical difference in the proportion of Lyve-1+ and CD68+ cells in the dorsal leptomeninges of mice of different ages （F=0.2686， P=0.9244， df=5）. Proportion of Lyve-1+ cells in the leptomeninges of 15-week-old APP/PS1 transgenic mice was lower than that of wild-type C57BL/6 mice （t=6.381，P=0.0078）. The ratio of Lyve-1+ cells was lower in the leptomeningeal of anti-Lyve-1-injected mice than that in the control group （MPBS=0.4513， Manti-Lyve-1=0.2692， q=8.726， P<0.0001）. The ratio of Lyve-1+ cells in the leptomeningeal of SAR131675-injected mice was lower than that in the control group （MSAR131675=0.3230， q=5.588， P=0.0006）. In the open field tests， SAR131675-injected mice showed reduced exploratory locomotion， but increased willingness to explore the central area. The anti-Lyve-1-injected mice showed an increased willingness to explore the central area. In the social interaction tests， the anti-Lyve-1-injected mice showed no reduction in social behavior or social preference. The SAR131675-injected mice showed reduced social behavior in terms of frequency of interaction but no social preference， suggesting that the SAR131675-injected mice had a social interaction decrease. In the novel object recognition tests， the anti-Lyve-1-injected mice showed no change in frequency， time and distance， indicating that the anti-Lyve-1-injected mice showed no change in short-term memory. The SAR131675-injected mice showed a decrease in short-term memory. ConclusionLeptomeningeal lymphatic endothelial cells play an important role in the early development of mice， which can be related to their phagocytosis of macromolecular substances.
