Astragalin Alleviates Neuronal Damage and Senile Plaque Deposition via Activating Autophagy in the Cortex of APP/PS1 Transgenic Mice
10.13471/j.cnki.j.sun.yat-sen.univ（med.sci）.2022.0209
- VernacularTitle:紫云英苷诱导自噬减轻APP/PS1转基因小鼠皮质内神经元损伤及老年斑沉积
- Author:
Cui-zhu YANG
1
;
Run-heng ZHANG
1
;
Shu-han WANG
1
;
Yu-yun JIANG
1
;
Jing LIU
1
;
Guo-ying LI
1
;
Yu-xin MA
1
Author Information
1. Department of Human Anatomy and Histology and Embryology， School of Life Sciences and Biopharmaceutics， Guangdong Pharmaceutical University， Guangzhou 510006， China
- Publication Type:Journal Article
- Keywords:
astragalin;
APP/PS1 transgenic mice;
autophagy;
neuronal damage;
Aβ deposition
- From:
Journal of Sun Yat-sen University(Medical Sciences)
2022;43(2):238-246
- CountryChina
- Language:Chinese
-
Abstract:
ObjectiveTo explore the effect of astragalin （AST） on neurons and Aβ plaques in the cortex of APP/PS1 transgenic mice. MethodsTwenty-four 8-month-old male APP/PS1 transgenic mice were randomly divided into APP/PS1 group， 10 mg/kg AST （APP/PS1+AST 10） group， and 20 mg/kg AST （APP/PS1+AST 20） group， 40 mg/kg AST （APP/PS1+AST 40） group， with 6 mice in each group. Six C57BL/6 male mice of the same age served as the control group （WT group）. AST drugs were continuously injected intraperitoneally for one month. Then Immunofluorescent staining was used to observe the deposition of Aβ plaques in the cortex. Nissl staining was used to observe the number and morphological changes of neurons in the cortex， and immunofluorescent multiple staining methods were used to observe the co-expression of LC3B， p62 and NeuN in the cortex. Then the expressions of NeuN， LC3B， and p62 protein were detected by Western blot method. ResultsImmunofluorescent staining results showed 20 mg/kg and 40 mg/kg AST reduced Aβ plaques deposition in the cortex of APP/PS1 mice （P < 0.000 1； P < 0.000 1）. Western blot analysis showed both 20 and 40 mg/kg AST increased the expression of NeuN protein in the cortex of APP/PS1 mice （P = 0.012 1； P < 0.000 1）. Immunofluorescent multiplex staining showed co-expression of LC3B， p62， and NeuN in the cortex of APP/PS1 mice. Western blot analysis showed AST increased the expression of LC3B （P = 0.007， P < 0.000 1） and decreased the expression of p62 （P < 0.000 1， P < 0.000 1） in the cortex of APP/PS1 mice. ConclusionsAST reduces neuronal damage and Aβ plaques deposition in the cortex of APP/PS1 mice by activating autophagy.
