Relationship between Cough Variant Asthma and Intestinal Flora Based on 16S rDNA Gene Sequencing
10.13471/j.cnki.j.sun.yat-sen.univ（med.sci）.2022.0408
- VernacularTitle:基于16S rDNA基因测序探讨咳嗽变异性哮喘与肠道菌群的关系
- Author:
Ling SHEN
1
;
Lin-lin ZENG
1
;
Qiao-mei LUO
1
;
Yi-lin FAN
1
;
Qian HE
1
;
Xiao-yun DUAN
2
Author Information
1. Life Science & Engineering College of South-west Jiao-tong University， Chengdu 610031，China
2. Sichuan Provincial Orthopedic Hospital， Chengdu 610041，China
- Publication Type:Journal Article
- Keywords:
cough variant asthma;
intestinal flora;
high-throughput sequencing;
exterior-interior relationship between the lung and the large intestine
- From:
Journal of Sun Yat-sen University(Medical Sciences)
2022;43(4):582-590
- CountryChina
- Language:Chinese
-
Abstract:
ObjectiveTo explore the effect of ovalbumin（OVA）-induced cough variant asthma （CVA） on the intestinal flora of mice， based on the traditional Chinese medicine theory of "exterior-interior relationship between the lung and the large intestine" and 16S ribosomal DNA gene sequencing technology. MethodsTwenty BALB/c female mice were randomly divided into model group and control group， and were sensitized and stimulated with OVA to establish a CVA mouse model. After modeling， the body weight and the cough response of mice were recorded by ammonia water-induced cough method. Hematoxylin-eosin staining was used to observe the pathological changes in the lungs of mice. The feces of mice were collected for 16S rDNA high-throughput sequencing analysis. ResultsCompared with the control group， the mice in the model group were in a poorer general state， being restless， scratching their ears， and losing weight （P<0.01）. The model group mice had increased cough times （P<0.01） and shortened cough latency （P<0.01） in comparison to the control group. Pathological results showed that the model group mice had obvious inflammatory infiltration， thickened airway smooth muscle， and epithelial cell necrosis. 16S ribosomal DNA sequencing showed that the species richness and diversity of the intestinal flora of mice in the model group increased， and the structure of the intestinal flora changed significantly. At the phylum level， the relative abundance of Firmicutes in the model group was decreased， and the relative abundance of Bacteroidetes and Euryarchaeota was increased. At the genus level， the relative abundance of Lactobacillus was decreased in the model group， and the relative abundance of Methanobrevibacter and Prevotellaceae UCG-003 was increased. ConclusionOVA-induced CVA mice had intestinal flora disorder， which provides new idea for the treatment of CVA， and further enriches the traditional Chinese medicine theory of “exterior-interior relationship between the lung and the large intestine”.
