Transcriptome Analysis of Campylobacter jejuni-Induced Colorectal Cancer
10.13471/j.cnki.j.sun.yat-sen.univ（med.sci）.2022.0405
- VernacularTitle:空肠弯曲杆菌诱导的结直肠癌的转录组学分析
- Author:
Yan LI
1
;
Yu-dan MAO
1
;
Xing-ding ZHANG
1
;
Xiang-yu MOU
1
Author Information
1. School of Medicine， Sun Yat-sen University， Shenzhen 518107， China
- Publication Type:Journal Article
- Keywords:
Campylobacter jejuni;
colorectal cancer;
transcriptomics
- From:
Journal of Sun Yat-sen University(Medical Sciences)
2022;43(4):548-562
- CountryChina
- Language:Chinese
-
Abstract:
ObjectiveTo explore the carcinogenic mechanism of Campylobacter jejuni. MethodsEighteen female C57BL/6 ApcMin/+ mice were randomly divided into the C. jejuni-infected group and the non-infection control group， each group with nine mice. Colorectal cancer of ApcMin/+ mice was induced by dextran sulfate sodium and gavage of C. jejuni （or PBS as a control）. At the end of the experiment， the number of tumors in colorectal tissues of mice in each group was counted， and RNA was extracted from colorectal tumors， along with para-cancer tissues as controls. Transcriptome sequencing was performed by RNA-Seq technology， and data were analyzed for differentially expressed genes （DEGs）. Further， selected DEGs were subjected to GO （gene ontology） enrichment analysis and KEGG pathway enrichment analysis. ResultsCompared with that of the non-infection control group， the incidence of tumor in C. jejuni-infected group was significantly higher （P < 0.01）， which indicated the success of recreating the C. jejuni-induced CRC model. RNA-seq results showed that there were 394 and 501 DEGs （fold change > 4 and P < 0.05） in the C. jejuni-infected group compared with the two control groups， respectively. In GO enrichment analysis， DEGs were mainly enriched in immune response regulation and activation pathways， multiple protein transport pathways and receptor binding pathways. Cancer-related pathways and metabolic pathways were significant enriched in KEGG pathway enrichment analysis. Among these DEGs， 17 genes were found in comparisons with both control groups. The 17 genes were further selected， resulting in 14 “core” DEGs. In further validation of qRT-PCR， 9 genes were significantly differentially expressed， among which 3 genes were up-regulated （Gm1987， Saxo1 and Plekhs1） and 6 were down-regulated （Lrp2， Serpina3c， Fabp4， Tmem52， Lrrn4 and Upk3b）. ConclusionThis study emphasizes 9 host genes that may play important and unique roles in the occurrence and development of colorectal cancer induced by C. jejuni， which provides new insights for further studies on the carcinogenic mechanism of C. jejuni.
