The Role and Mechanism of MiR-130b-5p in Down-regulating IGF-1 and Inhibiting the Migration and Invasion of Human Chorionic Trophoblast Cells
10.13471/j.cnki.j.sun.yat-sen.univ（med.sci）.2022.0404
- VernacularTitle:miR-130b-5p下调胰岛素样生长因子-1抑制人绒毛膜滋养层细胞迁移及侵袭的作用及机制
- Author:
Meng XIANG
1
;
Xu-dong ZHANG
2
Author Information
1. Department of Obstetrics and Gynecology, Clinical College, Xi 'an Medical University, Xi 'an 710021, China
2. Laboratory of Clinical Medicine, Xi 'an Medical University, Xi 'an 710021, China
- Publication Type:Journal Article
- Keywords:
human chorionic trophoblast cells;
insulin-like growth factor-1;
microRNA 130b-5p;
migration;
invasion
- From:
Journal of Sun Yat-sen University(Medical Sciences)
2022;43(4):539-547
- CountryChina
- Language:Chinese
-
Abstract:
ObjectiveTo explore the effects of microRNA 130b-5p （miR-130b-5p） on the migration and invasion of human chorionic trophoblast cells （HTR8/SVneo） by targeting insulin-like growth factor-1 （IGF-1）. MethodsHTR8/SVneo cells were divided into control group， miR-NC group， miR-130b-5p mimics group， miR-130b-5p mimics+pcDNA3.1 group and miR-130b-5p mimics+pcDNA3.1-IGF-1 group. The dual luciferase reporter gene was used to detect the targeting relationship between miR-130b-5p and IGF-1； real-time fluorescent quantitative PCR was used to detect the expression level of miR-130b-5p and IGF-1in HTR8/SVneo cell samples； the CCK-8 method was used to detect the proliferation of HTR8/SVneo cells； holographic cytometer and Transwell method was used to analyze the migration and invasion abilities of HTR8/SVneo cells； Western blot method was used to detect the expression of IGF-1， invasion， and epithelial-mesenchymal transition （EMT） proteins in HTR8/SVneo cell samples. ResultsThe results of the dual luciferase reporter gene showed that IGF-1 was a potential target gene of miR-130b-5p； compared with the miR-NC group， the expression level of miR-130b-5p， the cell proliferation inhibition rate， and the expression level of E-cadherin increased significantly in the miR-130b-5p mimics group. The expression levels of IGF-1， c-Myc， Cyclin D1， migration distance， number of invaded cells， the expression levels of matrix metalloproteinase 9 （MMP9）， matrix metalloproteinase 2 （MMP2）， neural cadherin （N-cadherin） and Vimentin were significantly reduced （P<0.05）； compared with the miR-130b-5pmimics+pcDNA3.1 group， the cell proliferation inhibition rate and the expression level of E-cadherin in the miR-130b-5p mimics+pcDNA3.1-IGF-1 group were significantly reduced， while the expression levels of c-Myc and CyclinD1， migration distance， number of invasive cells， the expression levels of MMP9， MMP2， Vimentin and N-cadherin were significantly increased （P<0.05）. ConclusionThe overexpression of miR-130b-5p may inhibit the migration and invasion of HTR8/SVneo cells by inhibiting the expression of IGF-1.
