Intracellular ATP Concentrations of CD4 Cells Is Better than the CD4/CD8 Ratio for Assessing the Immune Statuses of TB Patients
10.13471/j.cnki.j.sun.yat-sen.univ（med.sci）.2022.0508
- VernacularTitle:CD4细胞内ATP浓度代替CD4/CD8比值用于评估结核感染患者的免疫状态
- Author:
Zhen-jie LIU
1
;
Dong-zi LIN
2
;
Yu-pei TU
3
;
Dan ZHOU
3
;
Yu-mei LI
4
;
Jian-rong LOU
3
;
Xiang YANG
3
;
Ning XU
1
Author Information
1. The Second Affiliated Hospital of Guangzhou University of Chinese Medicine, Guangzhou 510370, China
2. Foshan Fourth People’s Hospital, Foshan 528200,China
3. Leide Biosciences Co., Ltd, Guangzhou 510670, China
4. Dongguan Sixth People's Hospital, Dongguan 523000, China
- Publication Type:Journal Article
- Keywords:
Mycobacterium tuberculosis;
basal intracellular ATP;
CD4 T lymphocytes;
immune status
- From:
Journal of Sun Yat-sen University(Medical Sciences)
2022;43(5):748-756
- CountryChina
- Language:Chinese
-
Abstract:
ObjectiveTo investigate the feasibility of basal ATP concentration in CD4 T lymphocytes instead of CD4/CD8 ratio in assessing the immune status of tuberculosis-infected patients. MethodsIn this study， the CD4 and CD8 cells in the same volume of whole blood were separated by magnetic beads method， and the basal ATP concentration of CD4 and CD8 cells was calculated to evaluate the immune status of tuberculosis-infected patients. Quantiferon Monitor （QFM） assay was used as a control. ResultsOur study confirmed that traditional CD4/CD8 ratio andCD4 T cell counting methodswerenot suitable for TB patients. Using the basal ATP concentration of CD4 cells to monitor active tuberculosis patients and healthy people， when the cut-off value was 290.7×10-6 g/L， the sensitivity was 86.21%， and the specificity was 76.19%， and the difference was statistically significant （P<0.000 1）.【Conclussions】In this study， to the best of our knowledge， we have for the first time used the basal ATP concentration in CD4+ T lymphocytes to evaluate the immune status of tuberculosis-infected patients under non-external stimulation conditions. It is suggested that the basal ATP concentration of CD4+ T lymphocytes can be better used to evaluate the immune status of tuberculosis patients than CD4/CD8 ratio.
