Effect of Integrin-linked Kinase on Derivation of Neural Stem-like Cells from Mesenchymal Stem Cells
10.3969/j.issn.1006-9771.2014.11.007
- VernacularTitle:整合素连接激酶对脐带间充质干细胞向神经干细胞样细胞分化的作用
- Author:
Jianyu ZHOU
;
Wei SUN
;
Xin ZHANG
;
Jianjun WANG
;
Ze CAO
- Publication Type:Journal Article
- Keywords:
integrin-linked kinase, mesenchymal stem cells, proliferation, neural stem cell-like cell
- From:
Chinese Journal of Rehabilitation Theory and Practice
2014;20(11):1022-1027
- CountryChina
- Language:Chinese
-
Abstract:
Objective To investigate the effect of integrin-linked kinase (ILK) on inducing mesenchymal stem cells (MSCs) to neuron stem-like cell in vitro. Methods MSCs isolated from umbilical cord were transfected with plasmid DNA encoding green fluorescent protein (GFP) and GFP-fusion ILK, wild and mutant, respectively; and cultured in serum-free media with basic fibroblast growth factor, epidermal growth factor and B27. It was assessed with the proliferative activity and the efficiency of differentiation into neural stem-like cell. Results MSCs transfected with GFP-fusion wild ILK expressed ILK and kinase-dead mutant ILK stably. The activity of proliferation increased and more of them differentiated into neural stem-like cell, which express CD133 and Nestin validated by PCR and Western blotting, and more of wild than of mutant. Conclusion Integrin-linked kinase may play a positive role in MSC differentiation into neural stem-like cell, which may associate with the activity of kinase.
