Clinical Significance of Expression of p-AXL and Co-expression with c-MYC and/ or BCL-2 in DLBCL
- VernacularTitle:p-AXL 与c-MYC 和/或BCL-2 共表达在弥漫大B 细胞性淋巴瘤中的临床意义
- Author:
Na-bo MIAO
1
;
Qi-ming LI
1
;
Shu-hua LI
1
;
Zhao-ming NIE
1
;
Fen-fen ZHANG
1
;
Ting-sheng PENG
1
Author Information
1. Department of Pathology, The First Affiliated Hospital, Sun Yat-sen University, Guangzhou 510080, China
- Publication Type:Journal Article
- Keywords:
DLBCL;
p-AXL;
BCL-2;
c-MYC;
prognosis
- From:
Journal of Sun Yat-sen University(Medical Sciences)
2020;41(4):572-581
- CountryChina
- Language:Chinese
-
Abstract:
【Objective】 To investigate the expression and significance of phosphorylated receptor tyrosine kinase(p-AXL) in diffuse large B cell lymphoma(DLBCL), and the clinical value of its co-expression with BCL-2 and /or c-MYC split gene. 【Methods】 Totally 118 cases of DLBCL were collected in the First Affiliated Hospital of Sun Yat-sen University from 2012 to 2017, and prepared as tissue array. p-AXL, c-MYC and BCL-2 proteins were detected by immunohistochemistry, and c-MYC split gene was detected by FISH staining. 【Results】 p-AXL protein was stained on tumor cells’membrane or in the cytoplasm of DLBCL cells. p-AXL was expressed more commonly in Non-GCB type than in GCB type. The expression of p-AXL was significantly correlated with the chemotherapy effect(P<0.01), and the expression of c-MYC split gene or BCL-2 protein separately(P<0.01). Co-expression of p-AXL and c-MYC, or BCL-2, or both of them was significantly correlated with the Hans typing(P<0.01). The PFS of p-AXL positive patients was obviously lower than that of p-AXL negative patients(P<0.05). The OS of p-AXL positive patients was also lower than that of the negative patients, but the difference had no statistical significance(P>0.05). Univariate analysis showed that p-AXL and male, p-AXL and Non-GCB type, p-AXL and c-MYC co-expression, p-AXL and c-MYC and/or BCL-2 co-expression were the risk factors for PFS and OS in DLBCL patients(P<0.05). Multivariate analysis showed that p-AXL positive in male(P<0.05) and p-AXL/c-MYC co-expression(P<0.01) were independent risk factors for PFS and OS in DLBCL patients. 【Conclusions】 p-AXL protein is expressed in DLBCL, and its expression is significantly related to the expression of c-MYC and BCL-2. p-AXL expression is associated with lower chemotherapy response rate, shorter progression-free survival and shorter overall survival. p-AXL/c-MYC co-expression or p-AXL positive in male may be an independent prognostic factor for DLBCL patients.
