Effects of Temperature on 18F-FDG Micro-PET/CT Imaging of Brown Adipose Tissue in Tumor Mice
- VernacularTitle:温度对乳腺癌小鼠棕色脂肪18F-FDG Micro-PET/CT成像的影响
- Author:
Qing-qiang TU
1
Author Information
1. Laboratory Animal Center, Sun Yat-sen University, Guangzhou 510080, China
- Publication Type:Journal Article
- Keywords:
Micro-PET/CT;
temperature;
brown adipose tissue(BAT);
18F-fluoro-deoxyglucose(18F-FDG);
maximum standardized uptake values(SUVmax)
- From:
Journal of Sun Yat-sen University(Medical Sciences)
2020;41(4):558-562
- CountryChina
- Language:Chinese
-
Abstract:
【Objective】 To investigate the effects of different temperature on 18F-fluoro-deoxyglucose(18F-FDG) uptake in brown adipose tissue(BAT) of tumor mice with use of Micro-PET/CT imaging. 【Methods】 After fasted for 8 h, all the four BALB/c breast cancer model mice were successively exposed to 3 different temperature together at 30℃, 25℃ and 20℃ respectively. Under each exposure, the mice were injected intravenously with 18F-FDG and 45 min later imaged by micro PET/CT. Then the images were reviewed and the maximum standardized uptake values(SUVmax) of BAT in the deep neck and scapula were measured. 【Results】 At the temperature of 30 ℃, 25 ℃ and 20 ℃, the SUVmax of 18F-FDG uptake in BAT were 0.63±0.16, 1.29±0.17 and 2.21±0.11, respectively; in tumor tissues 2.36±0.11, 1.85±0.10 and 1.51±0.17, respectively; in brain tissues 2.35±0.19, 2.34±0.14 and 2.38±0.11, respectively. 【Conclusion】 At room temperature(20℃), the metabolism in BAT is significantly enhanced, the metabolism in tumor tissues is induced. When temperature increases to 25℃ or 30℃, the uptake in non-specific areas such as brown fat in mice is significantly reduced or eliminated, and the metabolism in tumor tissues is enhanced. Temperature has significant effects on 18F-FDG uptake of BAT in mice and temperature control can help better 18F-FDG Micro-PET/CT imaging for tumor mice.
