Therapeutic Effects of Intraperitoneal Injection of Tetrahydrocurcumin against Allergic Asthma in Ovalbumin-induced Murine Model
- VernacularTitle:腹腔注射四氢姜黄素对卵清蛋白诱导过敏性哮喘小鼠的治疗作用
- Author:
Yin-fan WU
1
,
2
,
3
;
Qin LI
3
,
4
,
5
;
Jin-chao ZOU
3
,
4
,
5
;
Yu-heng MAO
3
,
4
,
5
;
Xing-yue GUO
1
,
2
,
3
;
Yanqiu CHEN
6
;
Yan YANG
3
,
4
,
5
Author Information
1. School of Public Health, Sun Yat-sen University, Guangzhou 510080, China
2. Guangdong Provincial Key Laboratory of Food, Nutrition and Health, School of Public Health, Sun Yat-sen University(Guangzhou Campus), Guangzhou 510080, China
3. Guangdong Engineering Technology Research Center of Nutrition Translation, Guangzhou 510080, China
4. Guangdong Provincial Key Laboratory of Food, Nutrition and Health, School of Public Health, Sun Yat-sen University(Guangzhou Campus), Guangzhou 510080, China
5. School of Public Health(Shenzhen), Sun Yat-sen University, Shenzhen 518000, China
6. Department of Otolaryngology, Guangzhou Women and Children Medical Centre, Guangzhou 510623, China
- Publication Type:Journal Article
- Keywords:
tetrahydrocurcumin;
allergic asthma;
therapy;
intraperitoneal injection;
inflammatory response
- From:
Journal of Sun Yat-sen University(Medical Sciences)
2020;41(4):525-533
- CountryChina
- Language:Chinese
-
Abstract:
【Objective】 To study the therapeutic effects of tetrahydrocurcumin(THC) via intraperitoneal(i.p.) injection on the respiratory symptoms and inflammatory responses in asthmatic mice. 【Methods】 BALB/c mice were randomly divided into 4 groups, including normal control group, ovalbumin(OVA) -induced model group, positive group and THC administration group. The latter two groups were treated with 2.5 mg/kg of dexamethasone(DEX) or 20 mg/kg of THC via i.p. injection from Day 21 to Day25. The weight changes and nasal symptoms were recorded before and after OVA challenge. The mice were sacrificed at the end of the experiment and the lung tissue and broncho-alveolar lavage fluid(BALF) were collected for assessment of histopathological alterations, Th cell subsets and related cytokine level. 【Results】 After THC treatment via i.p. injection, the asthmatic mice’s rubbing frequencies were reduced(P < 0.05) with a difference of 5.2 between the two means(95% CI = 0.66 to 9.74), lungs’pathological scores were reduced(P < 0.05) with a difference between the two means being 1.6(95% CI = 0.32 to 2.88), mucus production induced by hyperplasia of goblet cells was alleviated(P < 0.0001) with a difference between the two means being 9.56(95% CI = 5.05 to 14.07). Besides, compared with OVA group, the percentages of Th2 and Th17 cells were reduced(P < 0.01) with a difference between the two means being 1.49 and 2.15(95% CI = 0.50 to 2.49 and 0.72 to 3.58), respectively. The levels of interleukin(IL) -4, IL-5, IL-13 and IL-17A in BALF were decreased(P < 0.05) with a difference between the two means being 5.45, 4.13, 5.17 and 2.44 (95% CI = 1.95 to 8.94, 1.08 to 7.19, 0.80 to 9.54 and -0.30 to 5.17), respectively. The further comparison between THC and DEX groups showed no significant difference(P > 0.05) in the lung pathological change, Th17 cells, IL-13, and IL-17A levels. 【Conclusions】 It is concluded that i.p. injection of THC could effectively alleviate the respiratory symptoms and inhibit inflammatory reaction in asthmatic mice in short time with high safety. Therefore, i.p. injection of THC has the potential value to be the alternative therapeutic strategy for asthma.
