Cancer Associated Fibroblasts Promote Migration and Invasion of Gastric Cancer Cells by Inducing Epithelial-mesenchymal Transition
- VernacularTitle:癌相关成纤维细胞促进胃癌细胞迁移与侵袭
- Author:
Chang ZHOU
1
;
Wei-qiang CHEN
2
;
Yu-heng WANG
1
;
Xiao-xia YANG
1
;
Xing TU
1
;
Jing LIU
1
;
Li LUO
1
Author Information
1. School of Biosciences & Biopharmaceutics, Guangdong Pharmaceutical University, Guangzhou 510006, China
2. School of Nursing, Guangdong Pharmaceutical University, Guangzhou 510006, China
- Publication Type:Journal Article
- Keywords:
cancer associated fibroblasts(CAFs);
gastric cancer(GC);
migration;
invasion;
epithelial mesenchymal transition(EMT)
- From:
Journal of Sun Yat-sen University(Medical Sciences)
2020;41(4):501-508
- CountryChina
- Language:Chinese
-
Abstract:
【Objective】 To explore the effects of cancer associated fibroblasts(CAFs) on migration and invasion of Gastric cancer(GC) cells and the underlying mechanism. 【Methods】 The primary CAFs and the normal fibroblasts (NFs) were isolated from GC tissues and their matched adjacent normal gastric mucosa tissue respectively (n=3). The conditioned medium from cultured NFs(NFs-CM) and CAFs(CAFs-CM) were obtained and then incubated with the GC cell lines AGS and MGC803. GC cell migration and invasion were determined by wound healing assay and Transwell assay. Light microscopy was performed to observe the morphological changes of GC cells. Quantitative realtime PCR was employed to detect the changes in expression of epithelial mesenchymal transition(EMT) associated markers in GC cells. 【Results】 CAFs and NFs were successfully cultured and identified. Compared with NFs-CM, CAFs-CM accelerated migration and invasion of GC cells(all P<0.05); showed more shuttle-like or spindle-like shape of GC cells; revealed decreased expression of E-cadherin, increased expression of Vimentin and N-cadherin in GC cells(all P<0.05) . 【Conclusion】 CAFs could enhance the migration and invasion of GC cells AGS and MGC803 via regulating EMT.
