MicroRNA-204-3p Regulates Non-small Cell Lung Cancer Cells via Targeting EphB2
- VernacularTitle:MiR-204-3p 靶向EphB2 调控非小细胞肺癌的作用机制
- Author:
Chuan-hua XIE
1
;
Hai-long CHEN
1
;
Ping HUANG
1
;
Shou-jun GUO
1
;
Yi-lian QIU
1
;
Shuo WANG
1
;
Yi-yun PAN
1
;
Zhi-ping HU
2
Author Information
1. Department of Internal Medicine, Ganzhou Cancer Hospital, Jiangxi Province, Ganzhou 341000, China
2. Department of Physiology, Gannan Medical College, Ganzhou 341000, China
- Publication Type:Journal Article
- Keywords:
miR-204-3p;
EphB2;
non-small cell lung cancer;
proliferation;
apoptosis;
invasion;
migration
- From:
Journal of Sun Yat-sen University(Medical Sciences)
2020;41(5):716-725
- CountryChina
- Language:Chinese
-
Abstract:
【Objective】 To explore the regulatory mechanism of miR-204-3p targeting EphB2 gene on proliferation, apoptosis and invasion of NSCLC. 【Methods】 A549 cells were cultured and divided into 5 groups: NC mimic group, miR-204-3p mimic group, OE NCgroup, OE EphB2 group, miR-204-3p mimic+ OE EphB2 group. MTT, flow cytometry, Transwell and scratch test were used to detect cell proliferation, cycle, apoptosis, invasion and migration. Double Luciferase Report was used to analyze the targeting relationship between mir-204-3p and EphB2. The mRNA and protein expression of miR-204-3p, EphB2 were detected by qPCR and WB. 【Results】 Innon-small lung cancer cells, the binding site of mir-204-3p and EphB2 3'UTR region, the high expression of miR-204-3p significantly inhibited the expression of EphB2 mRNA and protein(P<0.01). Compared with the Negtively control group(NC mimic group), the proliferation of A549 cells in miR-204-3p mimicgroup were significantly decrease(P<0.05), and the apototic rate was significantly increased(P<0.05). Also, the cell migration and invasion ability were also decreased significantly(P<0.05). Transfection of Ephb2 reversed the above changes. In addition, in non-small cell lung cancer tissues, miR-204-3p was negatively correlated with EphB2 expression(r=0.636, P<0.001), and the overall survival was shorter in EphB2 high expression groups than that in low expression groups(logrank χ2=3.899, P=0.049) . 【Conclusion】 MiR- 204- 3p inhibits proliferation, migration and invasion of non-small lung cancer cells and induces apoptosis by down-regulating EphB2.
