Effects of Oxidative Stress in Follicular Environment on Embryo Quality in Women of Different Ages
- VernacularTitle:不同年龄女性卵泡液及颗粒细胞的氧化应激状态对胚胎质量的影响
- Author:
Can-xin WEN
1
;
Rui-qi LI
1
;
Song-bang OU
1
;
Dong-zi YANG
1
;
Yu LI
1
Author Information
1. Reproductive Center, Sun Yat-sen Memorial Hospital, Sun Yat-sen University, Guangzhou 510120, China
- Publication Type:Journal Article
- Keywords:
follicular fluid;
superoxide dismutase(SOD);
ovarian aging;
blastocyst rate;
granulosa cells
- From:
Journal of Sun Yat-sen University(Medical Sciences)
2020;41(3):465-472
- CountryChina
- Language:Chinese
-
Abstract:
【Objective】 To investigate the oxidative stress status in follicular fluid and granulosa cells obtained during oocyte retrieval cycles among women of different ages and to explore the relationship between the oxidative stress and the embryo quality. 【Methods】 From September 8, 2018 to January 18, 2019 in our reproductive center, 70 follicular fluid samples on the day of oocyte retrieval were collected from infertile women undergoing in vitro fertilization(IVF) or intracytoplasmic sperm injection(ICSI). We divided the samples into those at or above(aged group, n = 34) and below the age of 35(young group, n = 36) and compared seven oxidative stress markers in follicular fluid and gene expression of antioxidant enzymes in granulosa cells, and then analyzed their differences between groups and their relationship with embryo development parameters. 【Results】 We observed significantly higher concentration of hydrogen peroxide(H2O2) [155.84(137.11~199.30) vs. 66.94(51.05~95.42) μmol/L, P = 0.001] , significantly lower total superoxide dismutase (SOD) activity [0.69(0.55~0.85) vs. 0.83(0.76~0.94) U, P = 0.005] , decreased catalase(CAT) and glutathione reductase(GSR) expression(P<0.05) in women with advanced materal age. Total SOD activity in follicular fluid was statistically higher both in patients with normal fertilization rate(≥60%) and with blastocyst formation rate(≥ 60%) [0.82 (0.71~0.91) vs. 0.67(0.55~0.80) U, P = 0.007, 0.83(0.78~0.90) vs. 0.73(0.60~0.89) U, P = 0.049] . Multivariate logistic analysis revealed that higher total SOD activity correlated with normal fertilization rate(≥ 60%) and blastocyst formation rate(≥60%), with 5.478 and 3.383 for odds ratio(OR) values, 1.721~17.433 and 1.111~10.299 for 95% CI, 0.004 and 0.032 for P values, respectively. 【Conclusions】 The oxidative stress in follicular environment among women with advanced materal age is mainly characterized by increased level of reactive oxygen species(ROS), decreased SOD activity in follicular fluid and reduced expression of CAT and GSR in granulosa cells. Moreover, total SOD activity in follicular fluid was positively related to normal fertilization rate and blastocyst rate, which suggests that it may have a certain effect on the quality of oocytes.
