Expressions and Clinical Implication of UBE2 Family and UBE2T Gene in Lung Adenocarcinoma Based on Bioinformatics
- VernacularTitle:基于生物信息学途径评估UBE2家族及UBE2T基因在肺腺癌中的表达及临床意义
- Author:
Hao-shuai YANG
1
;
Hui-na LAI
1
;
Yi-ru RAO
1
;
Xiao-ping LIN
2
Author Information
1. Zhongshan School of Medicine, Sun Yat-sen University, Guangzhou 510080, China
2. Department of Nuclear Medicine, Cancer Center, Sun Yat-sen University, Guangzhou 510060, China
- Publication Type:Journal Article
- Keywords:
lung adenocarcinoma;
UBE2T;
UBE2 family;
gene expression;
database;
ubiquitination;
glucose metabolism
- From:
Journal of Sun Yat-sen University(Medical Sciences)
2020;41(3):445-451
- CountryChina
- Language:Chinese
-
Abstract:
【Objective】 To investigate the expressions of ubiquitin-conjugating enzyme(UBE2) family and ubiquitin-conjugating enzyme E2T(UBE2T) gene and their relationships with the clinicopathology in lung adenocarcinoma. 【Methods】 Based on Linkedomics database, the expression levels of UBE2C, UBE2M, UBE2S, UBE2T and UBE2V2 mRNA and their associations with the clinical characteristics of lung adenocarcinoma were collected and detected. Kruskal-Wallis H test was applied to compare the differences of pathologic stage, T stage and N stage, Wilcoxon rank sum test for M stage and Cox regression test for overall survival(OS). The expression profiles of UBE2T gene in lung adenocarcinoma and paracancerous tissues were obtained from the Cancer Genome Atlas(TCGA) database and then analyzed with independent samples t test. The Kyoto Encyclopedia of Genes and Genomes(KEGG) pathway and the enrichment were examined by using Gene Set Enrichment Analysis(GSEA) software. 【Results】 The expressions of UBE2C, UBE2M, UBE2S, UBE2T and UBE2V2 mRNA correlated with the clinicopathological features of lung adenocarcinoma. Compared with low UBE2T expression, high UBE2T expression predicted shorter OS time and advanced TNM stage. Their differences were statistically significant(P < 0.05). The UBE2T mRNA expression level was significantly upregulated in lung adenocarcinoma(P < 0.01). The gene enrichment analysis showed that the overexpression of UBE2T was closely related to 36 signal pathways(FDR < 0.05), including cell cycle(FDR = 0), spliceosome(FDR = 0), DNA repair(FDR = 0) and the glucose metabolism. The expressions of UBE2C, UBE2M, UBE2S, UBE2T and UBE2V2 mRNA in UBE2 family correlated with the clinicopathological features of lung adenocarcinoma. UBE2T was further identified as a factor most closely related with pathological grade, prognosis in lung adenocarcinoma and glycolysis process of tumor cells. 【Conclusion】 UBE2 family correlates with clinicopathological features of lung adenocarcinoma. UBE2T mRNA could be a new biomarker for lung adenocarcinoma.
