Protective Effect of Tri-Block Polymer Loaded with Phycocyanin on Oxidative Damage of Human Lens Epithelial Cells
- VernacularTitle:三嵌段聚合物搭载藻蓝蛋白对人晶状体上皮细胞氧化损伤的保护作用
- Author:
Meng-ting CHEN
1
;
Li-li HAO
1
;
Lian LIU
1
;
Tian-feng CHEN
2
;
Jing-xiang ZHONG
1
Author Information
1. Department of Ophthalmology, The First Affiliated Hospital of Jinan University, Guangzhou 510630, China
2. Department of Chemistry, Jinan University, Guangzhou 510630, China
- Publication Type:Journal Article
- Keywords:
phycocyanin;
triblock polymer;
lens epithelial cells;
oxidative damage;
apoptosis
- From:
Journal of Sun Yat-sen University(Medical Sciences)
2020;41(3):389-396
- CountryChina
- Language:Chinese
-
Abstract:
【Objective】 To observe the effect of tri-block polymer loaded with phycocyanin(PPP-PC) on the morphology and viability of human lens epithelial cells(HLEC) after oxidative damage induced by 2, 2'-azobis-2-methylpropanimidamide( AAPH), investigate PPP-PC's role in this process and tentatively explore the potential mechanism. 【Methods】 We constructed and purified PPP-PC, and established AAPH induced oxidative damage model of HLEC. There were three groups divided: normal control group, AAPH damage group and PPP-PC+AAPH treatment group. The inverted fluorescence microscope was employed to examine the cell morphology and viability, flow cytometry after annexin V-FITC/PI double staining was used to detect cell apoptosis rates and western blotting was utilized to determine the expression levels of apoptosis related proteins such as Bax, Bcl-2 and Caspase-3. 【Results】 Fluorescence microscope showed that in PPP-PC+AAPH group, the cell morphology was similar to that in control group and the cell viability was higher than that in AAPH group.The percentage of apoptotic cells in PPP-PC+AAPH group was 11.70%(10.63% , 12.17%), significantly lower than 32.35%(30.17%, 33.42%) in AAPH group(P < 0.05) .Compared with those in AAPH group, Bcl-2 expression increased, but Bax and Caspase-3 expression decreased in PPP-PC+AAPH group(P < 0.05) . 【Conclusions】 PPP-PC can protect AAPH induced oxidative damage of HLEC and inhibit cell apoptosis, which may be achieved by down-regulating the Bax/Bcl-2 ratio and inhibiting the activation of Caspase-3.
