Effect of Periostin on Neutrophil Regulation in Contact Hypersensitivity
- VernacularTitle:Periostin 对接触性超敏反应中的中性粒细胞调控作用的影响
- Author:
Zhong-yuan ZHANG
1
;
Mei-hua JIANG
1
Author Information
1. Center for Stem Cell Biology and Tissue Engineering, Zhongshan School of Medicine, Sun Yat-sen University, Guangzhou 510275, China
- Publication Type:Journal Article
- Keywords:
Periostin;
inflammatory response;
neutrophils;
cell infiltration
- From:
Journal of Sun Yat-sen University(Medical Sciences)
2020;41(3):363-369
- CountryChina
- Language:Chinese
-
Abstract:
【Objective】 To investigate the role of Periostin in regulating neutrophils at the early stage of swelling ear model. 【Methods】 Thirty animals were selected in each group and ear sensitization induced by DNFB(1-fluoro-2, 4-dinitrobenzene) sensitizer in wild type mice(WT) and Periostin-/- mice(POSTN-/-), respectively. After 24 h modeling, the auricle thickness was measured using a spiral micrometer. Immunohistochemical staining and flow cytometry were used to detect inflammatory cells and neutrophil infiltration in the auricle tissue. Real-time fluorescence quantitative PCR(qPCR) analysis was used to measure the expression of the neutrophil related cytokines and chemokines. 【Results】 Before the sensitizer-induced ear swelling model, the ear thickness of the two groups of mice was not statistically different(P > 0.05); and 24 hours after sensitization, the degree of ear swelling was greater in the POSTN-/- group than in the WT group (P < 0.05); HE staining showed that the number of inflammatory cells infiltrated in the POSTN-/- group was greater than that in the WT group(both P < 0.05); immunohistochemical staining and flow cytometry analysis showed that the neutrophil infiltration ratio in ear tissue was higher in POSTN-/- group than in WT group(all P < 0.05); both qPCR detected neutrophil- associated cytokine and chemokine expression levels in ear tissues were higher in POSTN-/- group than in WT group(all P < 0.05) . 【Conclusion】 Periostin reduced the infiltration of neutrophils in the early stage of inflammatory reaction of mouse ear swelling, which may play a role in inhibiting the development of inflammatory response.
