Rapid analysis and determination of the fragmentation regularity of phospholipids in human plasma based on UHPLC/Q-TOF-MS
10.16438/j.0513-4870.2022-0627
- VernacularTitle:基于UHPLC/Q-TOF-MS技术的人血浆磷脂类成分快速分析及质谱裂解规律初探
- Author:
Xun-long ZHONG
1
;
Ruo-lun WANG
1
;
Li-shi CHEN
2
;
Yan-mei ZHONG
2
Author Information
1. Department of Pharmacy, the Second Affiliated Hospital of Guangzhou Medical University, Guangzhou 510260, China
2. New Drug Research and Development Center of Guangdong Pharmaceutical University, Guangzhou 510006, China
- Publication Type:Research Article
- Keywords:
plasma phospholipid;
UHPLC/Q-TOF-MS;
phosphatidylcholine;
sphingomyelin;
ceramide;
fragmentation regularity
- From:
Acta Pharmaceutica Sinica
2022;57(10):3214-3222
- CountryChina
- Language:Chinese
-
Abstract:
Ultra-high performance liquid chromatography-tandem quadrupole time-of-flight mass spectrometry (UHPLC/Q-TOF-MS) was applied to rapidly identify the phospholipids in human plasma and explore the mass spectrometric fragmentation pattern. An acquity UHPLCTM BEH C18 column (50 mm × 2.1 mm, 1.7 μm) was utilized and eluted with a gradient system; the mobile phase consisted of 10 mmol·L-1 ammonium formate aqueous solution-0.1% formic acid aqueous solution (A) and acetonitrile-isopropanol (1∶1) organic solution (B) containing 10 mmol·L-1 ammonium formate-0.1% formic acid. The flow rate was 0.3 mL·min-1 and the column temperature was set at 50 ℃. An electrospray ionization (ESI) source was used to collect mass spectra in positive and negative ion mode. Based on the precise relative molecular weight and elemental composition calculated by Masslynx 4.1 software, comparison with references, and secondary mass spectrometry fragment ions and lipid databases, a total of 82 plasma lipids were identified, including 14 lysophosphatidylcholines (LysoPCs), 39 phosphatidylcholines (PCs), 17 sphingomyelins (SMs), 7 ceramides (Cers), 4 phosphatidylethanolamines (PEs), and 1 phosphatidylinositol (PI). A simple, efficient, fast and stable analytical method was established in this study for the qualitative analysis of phospholipids in human plasma, and the fragmentation regularity of the main phospholipids was determined. This work provides a good foundation for further metabolomics studies of plasma phospholipids. This study was approved by the Second Affiliated Hospital of Guangzhou Medical University Clinical Research and Application Institutional Review Board Approval (No. 2020-hs-07).
