Establishment of a method for the determination of four volatile components in Compound shexiang xuelian liuzhi plaster by GC
- VernacularTitle:复方麝香雪莲柳酯贴膏中4种挥发性成分含量测定方法的建立
- Author:
Gang ZHOU
1
;
Lianlian SHAN
1
;
Fangyuan MA
1
;
Bingyang CHAI
1
;
Huilan LEI
1
;
Hong TAO
1
;
Hua YAN
1
Author Information
1. Uygur Medicine
- Publication Type:Journal Article
- Keywords:
Compound shexiang xuelian liuzhi plaster;
camphor;
menthol;
borneol;
methyl salicylate;
gas chromatography
- From:
China Pharmacy
2022;33(20):2498-2502
- CountryChina
- Language:Chinese
-
Abstract:
OBJECTIVE To establish a method for simultaneously determining the contents of camphor ，menthol，borneol and methyl salicylate in Compound shexiang xuelian liuzhi plaster . METHODS The test solution was prepared by reflux extraction with ethyl acetate ，and was determined by gas chromatography （GC）. A Shimadzu SH -Rtx-Wax capillary column was used as the chromatographic column ，and a flame ionization detector was used as the detector . The detector temperature and the injector temperature were both set at 200 ℃. The flow rate of carrier gas （nitrogen）was 2.0 mL/min，the separation ratio was 20∶1，and the sample size was 1.0 μL. RESULTS The linear ranges of camphor ，menthol，borneol（calculated by the sum of isoborneol and borneol）and methyl salicylate were 11.5-230.4，10.6-211.6，11.3-225.5，11.0-219.1 μg/mL（r＞0.999）. RSDs of the precision ， repeatability and stability （48 h）tests were all less than 4%. The average recoveries of the four components were 100.7%，99.7%， 98.9% and 100.7%（RSDs were 4.3%，2.9%，2.2%，3.7%，n＝9）. The contents of camphor ，menthol，borneol and methyl salicylate in two specifications of Compound shexiang xuelian liuzhi plaster were 16.8-19.5，4.6-6.0，9.8-11.9，6.9-8.2 mg/piece（7 cm×10 cm/piece），and 8.3-8.6，2.2-2.4，4.7-4.8，3.2-3.6 mg/piece （5 cm×7 cm/piece）. CONCLUSIONS The method is successfully established for simultaneous determination of four volatile components in Compound shexiang xuelian liuzhi plaster .
