Effects of the components of traditional Chinese medicine for invigorating qi and activating blood circulation on cognitive function of chronic cerebral ischemia model mice and its mechanism
- VernacularTitle:益气活血中药组分对慢性脑缺血模型小鼠认知功能的影响及机制
- Author:
Fuhua HAN
1
,
2
;
Jiangang LIU
3
;
Linjuan SUN
1
;
Nannan LI
1
;
Jie GUAN
4
;
Min ZHAN
1
,
5
;
Wenjie CHEN
1
Author Information
1. Dept. of Encephalopathy，Xiyuan Hospital，China Academy of Chinese Medical Sciences，Beijing 100091，China
2. School of Clinical Medicine，Beijing University of Chinese Medicine，Beijing 100029，China
3. Cardiovascular Disease Center，Xiyuan Hospital，China Academy of Chinese Medical Sciences，Beijing 100091，China
4. Dept. of Endocrinology，Qingdao Haici Medical Group，Shandong Qingdao 266033，China
5. School of Graduate，China Academy of Chinese Medical Sciences，Beijing 100700，China
- Publication Type:Journal Article
- Keywords:
the components of traditional Chinese medicine for invigorating qi and activating blood circulation;
total ginsenoside
- From:
China Pharmacy
2022;33(20):2466-2470
- CountryChina
- Language:Chinese
-
Abstract:
OBJECTIVE To investigate the effects of the compo nents of traditional Chinese medicine （TCM）（total ginsenoside，total ketone ester of Ginkgo biloba ，total glucoside of Crocus sativus ）for invigorating qi and activating blood circulation on cognitive function of mice with chronic cerebral ischemia （CCI）and its related mechanisms . METHODS CCI model was established by stimulating bilateral common carotid arteries of C 57BL/6J mice with temperature controlled current . Model mice were randomly divided into model group ，aspirin group （positive control 10 mg/kg）and TCM group （the components of TCM for invigorating qi and activating blood circulation 33 mg/kg），sham operation group was set up additionally ，with 16 mice in each group. Except that the mice in model group and sham operation group were given water intragastrically ，the mice in the other groups were given corresponding medicine liquid intragastrically，once a day ，for 8 weeks. The latency of step down and the number of step -down errors of mice in each group were detected ；the ultrastructure of neurovascular units（NVU）in cerebral cortex and hippocampus was observed ；the protein and mRNA expressions of vascular endothelial growth factor（VEGF）and α7 nicotinic acetylcholine receptors （α7nAChRs） in cerebral tissue were detected ；the contents of VEGF，angiopoietin 1 （Ang1） and basic fibroblast growth factor（bFGF）were detected . RESULTS Compared with model group ，the latency of step -down was significantly prolonged （P＜ 0.05），and the number of step -down errors was significantly reduced （P＜0.05）in TCM group . The ultrastructural abnormalities of NVU in cerebral cortex and hippocampus were significantly improved ，and the protein and mRNA expressions of VEGF and α7 nAChRs and the contents of VEGF ，Ang1 and bFGF in cerebral tissue were significantly increased （P＜0.05）. CONCLUSIONS The components of TCM for invigorating qi and activating blood circulation can improve CCI -induced cognitive dysfunction in mice，the mechanism of which may be associated with protecting NVU ，recovering ultrastructural abnormalities of injured NVU ， and regulating the expression of proteins closely related to NVU and memory function .
