Study on the toxicity of the extract from Miao medicine Wikstroemia indica to zebrafish
- VernacularTitle:苗药了哥王提取物对斑马鱼的毒性作用
- Author:
Zhengyan HE
1
;
Guo FENG
1
,
2
;
Chuanqi ZHENG
1
;
Wei LI
1
;
Wen LIU
1
;
Guanglin ZHU
1
;
Wenjing WANG
1
;
Hongmei SU
1
;
Xueli SONG
1
;
Ju ZHANG
1
Author Information
1. College of Pharmacy，Guizhou University of Traditional Chinese Medicine，Guiyang 550025，China
2. National Engineering and Technology Research Center of Miao Medicine，Guiyang 550025，China
- Publication Type:Journal Article
- Keywords:
Miao medicine;
extract form Wikstroemia
- From:
China Pharmacy
2022;33(20):2443-2447
- CountryChina
- Language:Chinese
-
Abstract:
OBJECTIVE To study the toxicity of the extract from Mi ao medicine Wikstroemia indica to zebrafish . METHODS Zebrafish embryo model was used as the object ，after exposure to W. indica extract （10，20，40 μg/mL），the number of spontaneous twitching within 1 min，heart rate within 10 s，the occurrence of malformation and death were detected and recorded . Zebrafish was used as the object ，after exposure to W. indica extract（10-100 μg/mL）for 24，48 and 72 h，and the median lethal concentration（LC50）of W. indica extract to zebrafish at different time points were calculated . After exposure to low ，medium and high concentration （27，37，51 μg/mL）of W. indica extract，the liver phenotype ，hepatocyte apoptosis and lipid deposition of zebrafish were observed ，and the activities of alanine aminotransferase （ALT），aspartate aminotransferase （AST）and lactate dehydrogenase（LDH）in liver tissue were detected . RESULTS Compared with blank group ，the number of spontaneous twitching ， malformation rate （except for 10 μg/mL group ）and mortality of embryos increased significantly in 10，20，40 μg/mL groups of W. indica extract，and the heart rate （except for 10，20 μg/mL group ）of embryos decreased significantly （P＜0.05）. LC50 of W. indica extract to zebrafish at 24，48 and 72 h were 39.850，28.300 and 21.490 μg/mL，respectively. After drug treatment ，the transparency of liver area of zebrafish in low ，medium and high concentration groups of W. indica extract reduced and their shape were enlarged；apoptosis and lipid deposition increased ；the activities of ALT ，AST and LDH in liver tissue were significantly increased （P＜0.05）. CONCLUSIONS Miao medicine W. indica extract had develop mental toxicity to zebrafish embryos and he patotoxicity to zebrafish .
