Ethanol extract from Euphorbia humifusa inhibits the malignant biological behaviors of colorectal cancer SW480 cells by regulating the circRHOT1/miR-29a-3p molecular axis
DOI：10.3872/j.issn.1007-385x.2022.09.004
- VernacularTitle:地锦草乙醇提取物通过circRHOT1/miR-29a-3p轴抑制结直肠癌SW480细胞的恶性生物学行为
- Author:
FAN Honggea
1
,
2
,
3
;
DAI Weibob
1
,
2
,
3
;
ZHANG Xuefengc
1
,
2
,
3
Author Information
1. a. Department of Anorectal Medicine
2. b. Laboratory of Pharmacology of Traditional Chinese Medicine
3. c. the Third Department of Surgery, Zhongshan Hospital of Traditional Chinese Medicine, Zhongshan 528400, Guangdong, China
- Publication Type:Journal Article
- Keywords:
结直肠癌；SW480细胞；地锦草；乙醇提取物；circRHOT1；miR-29a-3p；增殖；迁移；侵袭
- From:
Chinese Journal of Cancer Biotherapy
2022;29(9):806-812
- CountryChina
- Language:Chinese
-
Abstract:
[摘 要] 目的：探讨地锦草乙醇提取物（EEEH）对人结直肠癌SW480细胞生物学行为的影响及其分子机制。方法：体外培养SW480细胞，实验分为Con组、EEEH-L组、EEEH-M组、EEEH-H组、si-NC组、si-circRHOT1组、EEEH-H+pcDNA组、EEEH-H+pcDNA-circRHOT1组，分别以si-NC、si-circRHOT1、pcDNA、pcDNA-circRHOT1转染SW480细胞，采用CCK-8法、细胞克隆形成实验、Transwell实验分别检测转染后各组细胞的增殖、迁移及侵袭能力，qPCR法检测转染后各组SW480细胞circRHOT1和miR-29a-3p的表达，WB法检测各组细胞中MMP-2、MMP-9蛋白的表达。双荧光素酶报告基因实验检测circRHOT1与miR-29a-3p之间的靶向关系。结果：与Con组比较，EEEH-L组、EEEH-M组、EEEH-H组SW480细胞中MMP-2和MMP-9蛋白表达均明显降低（均P<0.05），circRHOT1的表达均降低（均P<0.05）而miR-29a-3p的表达均升高（均P<0.05）且呈剂量依赖性；细胞的存活率、细胞克隆形成数、迁移及侵袭细胞数均减少（均P<0.05）。circRHOT1可靶向负调控miR-29a-3p的表达。敲减circRHOT1可抑制SW480细胞的增殖、迁移及侵袭能力，而过表达circRHOT1则可减弱EEEH对SW480细胞增殖、迁移及侵袭的抑制作用。结论：EEEH可通过调控circRHOT1/miR-29a-3p轴而抑制结直肠癌SW480细胞的增殖、迁移及侵袭能力。
- Full text:20220904.pdf
