Expression and clinical significance of cold-induced RNA-binding protein in lung adenocarcinoma analyzed based on bioinformatics
- VernacularTitle:基于生物信息学分析冷诱导 RNA 结合蛋白在肺腺癌组织中的表达及临床意义
- Author:
Cui XIAO
1
;
Jianbao YANG
1
;
Cheng WANG
1
;
Yiming SUN
1
;
Xuan LI
1
;
Zheng LI
1
;
Yanbin LIU
1
;
Haiming AN
1
Author Information
1. Department of Thoracic Surgery, Lanzhou University Second Hospital, Lanzhou University Second Clinical Medical College, Lanzhou, 730030, P. R. China
- Publication Type:Journal Article
- Keywords:
Cold-induced RNA-binding protein (CIRBP);
lung adenocarcinoma;
bioinformatics analysis;
prognosis
- From:
Chinese Journal of Clinical Thoracic and Cardiovascular Surgery
2022;29(07):914-922
- CountryChina
- Language:Chinese
-
Abstract:
Objective To analyze the expression of cold-induced RNA-binding protein (CIRBP) in lung adenocarcinoma and its clinical significance based on bioinformatics, in order to provide a new direction for the study of therapeutic targets for lung adenocarcinoma. Methods The CIRBP gene expression data and patient clinical information data in lung adenocarcinoma tissues and adjacent tissues were downloaded from The Cancer Genome Atlas and Gene Expression Omnibus databases. The expression of CIRBP in lung adenocarcinoma was analyzed. Furthermore, its relationship with clinicopathological features and prognosis in patients with lung adenocarcinoma was analyzed. GO and KEGG enrichment analysis were carried out for the screened genes. The CIRBP protein interaction network was constructed by STRING, and the correlation analysis was carried out using the GEPIA online website. Results The expression level of CIRBP gene in lung adenocarcinoma tissues was significantly lower than that in adjacent tissues (P<0.01), and its expression level was correlated with T stage and N stage in clinicopathological features. The prognosis of patients with high CIRBP expression in lung adenocarcinoma was significantly better than that with low CIRBP expression. Univariate and multivariate Cox regression analysis showed that CIRBP was an independent prognostic factor in patients with lung adenocarcinoma. GO functional annotation showed its enrichment in organelle fission, nuclear fission, chromosome separation, and DNA replication, etc. KEGG analysis showed that it was mainly involved in cell cycle and DNA replication. Protein interaction network and GEPIA online analysis showed that the expression level of CIRBP was negatively correlated with the expression level of cyclin B2. Conclusion CIRBP gene is down-regulated in lung adenocarcinoma tissues, and its expression level is closely related to patient prognosis. CIRBP gene may be a potential therapeutic target and prognostic marker for lung adenocarcinoma.
