Effect of DUS4L knockdown on gene expression regulation of human A549 lung adenocarcinoma cell line and analysis of different genes
- VernacularTitle:敲减 DUS4L 对人 A549 肺腺癌细胞基因表达调控的影响及差异基因分析
- Author:
Jie LI
1
;
Zheng LI
1
;
Bin LI
1
;
Qiyao YU
1
;
Wenjie MAO
1
;
Yuqi MENG
1
;
Duojie ZHU
1
;
Haiming FENG
1
;
Ci YIN
1
;
Cui XIAO
1
Author Information
1. Department of Thoracic Surgery, Lanzhou University Second Hospital, Lanzhou University Second Clinical Medical College, Lanzhou, 730030, P. R. China
- Publication Type:Journal Article
- Keywords:
Dihydrouridine synthase 4-like;
lung adenocarcinoma;
RNA-seq;
differential genes;
bioinformatics analysis
- From:
Chinese Journal of Clinical Thoracic and Cardiovascular Surgery
2022;29(06):761-769
- CountryChina
- Language:Chinese
-
Abstract:
Objective To explore the mechanism of dihydrouridine synthase 4-like (DUS4L) on the development of lung adenocarcinoma (LUAD). Methods The RNA-seq expression data of LUAD was downloaded from The Cancer Genome Atlas (TCGA), and the relationship between its clinical pathological characteristics and DUS4L mRNA expression was evaluated. The effect of DUS4L knockdown on the proliferation of A549 cells was detected by EDU proliferation assay. The gene expression profile of lung adenocarcinoma A549 cells in the DUS4L knockdown group (KD group) and control group (NC group) was detected by transcriptome sequencing technique. The differential genes were screened by DESeq2. ClusterProfiler was used to perform GO functional enrichment analysis of differential genes. Results The expression of DUS4L mRNA in LUAD tissues was higher than that in normal tissues, and the up-regulation of DUS4L was related to the clinical pathological characteristics of LUAD patients. EDU proliferation assay suggested that knocking down DUS4L could inhibit the proliferation of A549 cells. A total of 456 differential genes were screened, including 289 up-regulated genes and 167 down-regulated genes [|log2(fold change)|>1 and Padj<0.05]. STC2 and TRIB3 were significantly down-regulated (P<0.05). Differential genes were mainly involved in the production of interleukin-8, angiogenesis, vascular endothelial cell proliferation and other biological pathways. Conclusion DUS4L can widely regulate the gene expression of LUAD cells, which provides a new idea for further studying the function and role of DUS4L in the occurrence and development of LUAD and finding new therapeutic targets for LUAD.
