Effect of Cx43 Protein on Improvement of Learning and Memory Ability Induced by Enriched Environment in Rats with Traumatic Brain Injury
10.3969/j.issn.1006-9771.2014.09.007
- VernacularTitle:Cx43 蛋白在丰富环境改善脑外伤大鼠学习记忆能力中的作用
- Author:
Shan JIANG
;
Yana LI
;
Hongping XIE
;
Huihui WANG
;
Ling LI
- Publication Type:Journal Article
- Keywords:
traumatic brain injury, Cx43 protein, learning and memory ability, enriched environment, rats
- From:
Chinese Journal of Rehabilitation Theory and Practice
2014;(10):924-927
- CountryChina
- Language:Chinese
-
Abstract:
Objective To explore the effect of Cx43 protein on improvement of learning and memory ability induced by enriched environment (EE) in rats with traumatic brain injury (TBI). Methods TBI model was made by fluid percussion injury (FPI) in Sprague-Dawley rats. The TBI rats were divided into EE group (A), standard housing (ST) group (B), Cx43 specific antisense oligonucleotides (Cx43 ASODN)+EE group (C) and scrambled sequence ODN+EE group (D) with 6 rats in each group. Another 6 normal rats were taken as the control group. Groups C and D were given hippocampal microinjection of Cx43-ASODN (2 μl/d/rat, 1.5 mmol/L) and ScrbASODN (2 μl/d/ rat, 1.5 mmol/L) respectively. Morris water maze was used to evaluated the learning and memory ability. Results The latency was longer and the traversing times was less in group B than in the control group (P<0.05). The latency was shorter in group A than in group B (P<0.05), and there was no significant difference between group A and the control group (P>0.05) from the 9th day after injury. The traversing times was more in group A than in group B and there was no significant difference between group A and the control group (P>0.05). The latency was longer and the traversing times was less in group C than in group A (P<0.05). Conclusion Cx43 protein may participate in the improvement of the learning and memory ability induced by EE in rats with TBI.
