Improvement effects of Huorongcaohuangqi admixture decoction on mesangial proliferative glomerulonephritis model rats and its mechanism
- VernacularTitle:火绒草黄芪混煎液对系膜增生性肾小球肾炎模型大鼠的改善作用及机制
- Author:
Xia HOU
1
;
Yue ZHAO
2
;
Ying ZHANG
2
;
Dong JIA
2
;
Kun ZHONG
3
;
Xiaonan FANG
3
;
Xiaobo DONG
3
;
Yue QI
1
Author Information
1. Dept. of Pharmaceutical Technology，Jiangsu Provincial Xuzhou Pharmaceutical Vocational College，Jiangsu Xuzhou 221116，China
2. Dept. of Pharmacology，the Second Affiliated Hospital of Liaoning University of TCM，Shenyang 110034，China
3. College of Graduate School，Liaoning University of Traditional Chinese Medicine，Shenyang 110847，China
- Publication Type:Journal Article
- Keywords:
Leontopodium leontopodioides;
Astragalus memeranaceus;
mesangial proliferative glomerulonephritis;
mesangial
- From:
China Pharmacy
2022;33(19):2359-2363
- CountryChina
- Language:Chinese
-
Abstract:
OBJECTIVE To study the improvement effects of Huorongcaohuangqi （HRCHQ） admixture decoction on mesangial proliferative glomerulonephritis （MsPGN）model rats and its mechanism . METHODS Totally 70 rats were divided into sham operation group （n＝10）and modeling group （n＝60）according to body weight . Sham operation group underwent sham operation，and MsPGN model was induced by immunological method （Freund’s adjuvant+BSA+lipopolysaccharide ）in modeling group. After successfully modeling ，50 rats were randomly divided into model group ，HRCHQ high -dose，medium-dose and low - dose groups （4.05，2.03，1.02 g/kg），Benazepril hydrochloride tablet group （20 mg/kg），with 10 rats in each group . Sham operation group and model group were given distilled water intragastrically ；other groups were given relevant medicine intragastrically 15 mL/kg，once a day ，for consecutive 5 weeks. After last administration ，the contents of total protein ，albumin and urea nitrogen were determined in the serum of rats . The expressions of transforming growth factor β1（TGF-β1）and tumor necrosis factor α（TNF-α）in renal tissue were detected by immunohistochemistry ；the expression of complement C 3 in renal tissue was detected by immunofluorescence . The phosphorylation level of phosphatidylinositol 3 kinase/protein kinase B/glycogen synthase kinase 3β（PI3K/Akt/GSK-3β） signal pathway related proteins and expression level of fibronectin （FN） in renal tissue were detected by Western blot . RESULTS Compared with model group，the serum contents of total protein and albumin were increased significantly in HRCHQ high -dose group （P＜0.05 or P＜0.01）. The serum content of urea nitrogen ， the E-mail：Houxia_75@163.com expressions of TGF -β1，TNF-α，FN and complement C 3，and the phosphorylation levels of PI 3K，Akt and GSK 3β protein were all decreased significantly （P＜0.05 or P＜0.01）. CONCLUSIONS HRCHQ can improve MsPGN by reducing the proliferation of mesangial cells and inhibiting the activity of PI 3K/ Akt/GSK3β signaling pathway .
