Mechanism of Suyu tongfeng prescription in the treatment of gouty arthritis through ROS/TXNIP/NLRP 3 signaling pathway
- VernacularTitle:苏萸痛风方通过ROS/TXNIP/NLRP3信号通路抗痛风性关节炎的作用机制
- Author:
Yuan CHENG
1
;
Li ZHANG
2
;
Yi TANG
1
;
Yimin TAN
1
;
Juan LI
1
;
Ying SONG
3
;
Qianghua YUAN
3
;
Xiaoyu TAN
4
Author Information
1. Dept. of Pharmacology，Hospital of Chengdu University of TCM，Chengdu 610072，China
2. Dept. of Respiratory Medicine，Chengdu Xindu District Hospital of TCM，Chengdu 610500，China
3. Dept. of Pharmacy，Hospital of Chengdu University of TCM，Chengdu 610072，China
4. Dept. of Clinical Laboratory，Hospital of Chengdu University of TCM，Chengdu 610072，China
- Publication Type:Journal Article
- Keywords:
Suyu tongfeng prescription;
gouty arthritis;
ROS/TXNIP/NLRP3 signaling pathway;
NLRP3 inflammasome;
rats
- From:
China Pharmacy
2022;33(19):2343-2347
- CountryChina
- Language:Chinese
-
Abstract:
OBJECTIVE To study the mechanism of Suyu tongfeng prescription against gouty arthritis . METHODS Male SD rats were randomly divided into normal control group ，model group ，colchicine tablets group （positive control drug ，0.3 mg/kg） and high -dose，medium-dose and low -dose groups （5，2.5，1.25 g/kg）of Suyu tongfeng prescription according to body mass ，with 10 rats in each group . The rats in the administration group were orally given the corresponding drugs once a day （10 mL/kg）for 7 consecutive days . Rats in the normal control group and model group were given equal volume of water intragastrically . On the 6th day，1 h after administration ，except for normal control group ，the rats in other groups were injected with sodium urate into the joint to replicate the gouty arthritis model . The degree of joint swelling and the score of inflammatory indexes were measured at 2， 6 and 24 h after the establishment of the model . One hour after the last administration ，the activity of oxidative stress related indicators [superoxide dismutase （SOD），xanthine oxidase （XOD），malondialdehyde（MDA）] and the levels of inflammatory factors [interleukin 1β（IL-1β），IL-18，tumor necrosis factor α were detected in the serum . The histopathological changes of ankle joints in each group were observed ；the expressions level of thioredoxin interacting protein （TXNIP）， mail：150120239@qq.com NOD like receptor thermoprotein domain related protein 3 （NLRP3），apoptosis related spot like protein （ASC）in the @qq.com ankle joint of rats were detected . RESULTS After intervened with Suyu tongfeng prescription ，the swelling degree of joints ，the score of inflammation indexes ，the edema of synovial tissue and the amount of inflammatory cells were reduced . The activity of SOD in Suyu tongfeng prescription high -dose group was increased significantly（P＜0.01），while the activities of XOD and MDA as well as the levels of IL -1β，IL-18 and TNF -α were all decreased significantly（P＜0.01）. The level of ROS and the protein expressions of TXNIP ，NLRP3 and ASC in ankle joint were all decreased significantly （P＜0.05 or P＜0.01）. The activities/levels of the above indexes were also significantly reversed in the middle-dose and low -dose groups of Suyu tongfeng prescription （P＜0.05 or P＜0.01）. CONCLUSIONS Suyu tongfeng prescription can inhibit the activation of NLRP 3 inflammasome through ROS/TXNIP/NLRP 3 signaling pathway ，and then play the role of anti -gouty arthritis .
