Inhibitory effects of eriocalyxin B on epithelial -mesenchymal transition in colon cancer cells through the Wnt/ β-catenin pathway
- VernacularTitle:毛萼乙素通过Wnt/β-catenin通路抑制结肠癌细胞上皮间质转化
- Author:
Wei KUANG
1
;
Yinbin ZHENG
2
;
Yuqi LI
1
;
Pingping TIAN
3
;
Qiang SU
4
;
Xiaocong XIANG
1
Author Information
1. Institute of Tissue Engineering and Stem Cells，Nanchong Central Hospital Affiliated to North Sichuan Medical College，Sichuan Nanchong 637000，China
2. Dept. of Cardio -Thoracic Surgery，Nanchong Central Hospital Affiliated to North Sichuan Medical College，Sichuan Nanchong 637000，China
3. School of Medical Technology，Anhui Medical College，Hefei 230601，China
4. Dept. of Pharmacy，Nanchong Central Hospital Affiliated to North Sichuan Medical College，Sichuan Nanchong 637000，China
- Publication Type:Journal Article
- Keywords:
eriocalyxin B;
colon cancer;
Wnt/β-catenin
- From:
China Pharmacy
2022;33(19):2326-2332
- CountryChina
- Language:Chinese
-
Abstract:
OBJECTIVE To study the effects and m echanism of eriocalyxin B （EriB）on epithelial -mesenchymal transition （EMT）of colon cancer cells . METHODS The colon cancer cells HT 29 and HCT 116 as research objects . Scratch-healing assay and Transwell assay were used to detect the effects of EriB on migration and invasion of two kinds of cells . Western blot method was used to detect the effects of EriB （1.0 μmol/L），EriB+Wnt/β-catenin signaling pathway agonist Licl （1.0 μmol/L EriB+ 10 mmol/L Licl）or EriB+Wnt/ β-catenin signaling pathway inhibitor XAV 939（1.0 μmol/L EriB+ 10 μmol/L XAV 939）on the expressions of EMT related proteins [E-cadherin，N-cadherin，Snail] and Wnt/ β-catenin signaling pathway related proteins [β-catenin，c-myc， cyclin D 1，TCF4]. RESULTS EriB can significantly inhibit the invasion and migration of two kinds of cells （P＜0.01）. EriB significantly reduced the protein expressions of N -cadherin，Snail，β-catenin，c-myc，cyclin D 1 and TCF 4 while increases the protein expression of E -cadherin（P＜0.05 or P＜0.01）. After EriB combined with Licl ，the protein expressions of β-catenin，c- myc，cyclin D 1 and TCF 4 in the two kinds of cells were reverse d（P＜0.05 or P＜0.01）. After combined with XAV 939，the protein expressions of β-catenin and N -cadherin were further down -regulated（P＜0.05 or P＜0.01）；the protein expression of E -cadherin was further up -regulated（P＜0.01），while migration rate was decreased significantly （P＜0.01）. CONCLUSIONS EriB can inhibit the epithelial -mesenchymal transition of colon 19SXHZ0351） cancer cells by inhibiting the activation of Wnt/ β -catenin
