Random Amplified Polymorphic DNA for Classification and Identification of Dermatophytes.
- Author:
Yeong Seon LEE
1
;
Jae Il YOO
;
Yeon Hwa CHOI
;
Hyung Yeul JOO
;
Bong Su KIM
;
Dong Han KIM
Author Information
1. Laboratory of Nosocomial Pathogens, National Institute of Health Seoul, Korea.
- Publication Type:In Vitro ; Original Article
- Keywords:
RAPD PCR;
Trichopyton;
Microsporum;
Epidermophyton
- MeSH:
Arthrodermataceae*;
Classification*;
Clinical Laboratory Techniques;
Diagnostic Tests, Routine;
DNA*;
Epidermis;
Epidermophyton;
Fungi;
Gelatin;
Hair;
Magnesium Chloride;
Microsporum;
Polymerase Chain Reaction;
Sensitivity and Specificity;
Taq Polymerase;
Tinea
- From:Korean Journal of Medical Mycology
1998;3(2):107-114
- CountryRepublic of Korea
- Language:Korean
-
Abstract:
BACKGROUND: Dermatophytoses are infections of keratinized tissues, that is, the epidermis, hair and nails, caused by a group of specialized fungi, the dermatophytes. Laboratory diagnoses of dermatophytes such as Tricophyton, Microsporum and Epidermophyton are made by microscopic examination and in vitro culture but they are either time consuming of lacking specificity. OBJECTIVE: In order to develop and apply more rapid and precise diagnostic tests for fungal pathogens to facilitate the improved identification of dermatophytes, we investigated random amplified polymorphism DNA for classification and identification of dermatophytes. METHODS: Amplification reactions were performed in volumes of 5011 containing 10mM Tris-HCl(pH 8.3), 50mM KCl, 1.5mM MgCl2, 0.01% (w/v), gelatin, 200mM dNTP mixture, 50pM primer, Taq polymerase (0.025 units/ microliter), DNA 0.001 microgram/microliter. The optimal condition to. PCR was 2 cycles (denaturing 94 degrees C 2min, annealing 33 degrees C 2min, extension 72 degrees C 4min), 40 cycles, and extension (72 degrees C 10min). RESULTS: RAPD showed interspecies polymorphism in but it had identical patterns in intraspecies. CONCLUSION: It was confirmed that RAPD PCR analysis with optimal conditions is a fast, economical and reproducible method for identification and classification of dermatophytes isolates.
